Anti-beta 2 Microglobulin抗体(ab6608)

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Key features and details

  • Rabbit polyclonal to beta 2 Microglobulin
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-beta 2 Microglobulin抗体
    参阅全部 beta 2 Microglobulin 一抗

  • 描述

    兔多克隆抗体to beta 2 Microglobulin

  • 宿主

    Rabbit

  • 种属反应性

    与反应: Human

  • 免疫原

    b 2-Microglobulin [Human Urine]

  • 常规说明

    The antibody reacts with b2-microglobulin isolated from human urine showing a 12.8 kDa band under non-reducing condition of SDS-PAGE.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

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    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...

  • 纯度

    IgG fraction

  • 纯化说明

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified b 2-Microglobulin [Human Urine].

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

靶标

  • 功能

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

  • 疾病相关

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

  • 序列相似性

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • 翻译后修饰

    Glycation of Ile-21 is observed in long-term hemodialysis patients.

  • 细胞定位

    Secreted. Detected in serum and urine.
  • Information by UniProt

  • 数据库链接

    • 别名

      • B2M antibody
      • B2MG_HUMAN antibody
      • Beta 2 microglobin antibody
      • Beta 2 microglobulin antibody
      • Beta 2 microglobulin precursor antibody
      • Beta chain of mhc class 1 proteins antibody
      • Beta chain of MHC class I molecules antibody
      • Beta-2-microglobulin form pI 5.3 antibody
      • CDABP0092 antibody
      • Hdcma22p antibody
      • IMD43 antibody
      see all

    文献 (4)

    发表研究结果有使用 ab6608？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6608 被引用在 4 文献中.

    • Kotsiou E  et al. Dimerization of soluble disulfide trap single-chain major histocompatibility complex class I molecules dependent on Peptide binding affinity. Antioxid Redox Signal 15:635-44 (2011). WB ; Human . PubMed: 21050141
    • Vecchi C  et al. Huh-7: a human "hemochromatotic" cell line. Hepatology 51:654-9 (2010). ICC/IF ; Human . PubMed: 20017200
    • Pi M  et al. GPRC6A null mice exhibit osteopenia, feminization and metabolic syndrome. PLoS ONE 3:e3858 (2008). WB ; Human . PubMed: 19050760
    • Yalcin, A and Cetin, M Electrophoretic Separation of Urine Proteins of Healthy Dogs and Dogs with Nephropathy and Detection of Some Urine Proteins of Dogs Using Immunoblotting. Revue Med. Vet. 155:104-112 (2004). PubMed:

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    1-3 of 3 Abreviews or Q&A

    Question

    ADDITIONAL NOTES I only would like to know which size i am suposed to detect in western blotting with this antibody. Is b2microglobulin coupled to 44kDa HLA chain to form a complex about 56kDa? Is 44kDa the size of the complex detected with this antibody? Thank you

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    Answer

    Thank you for yur enquiry and your interest in our products. The antibody reacts with b2-microglobulin isolated from human urine showing a 12.8 kDa band under non-reducing condition of SDS-PAGE.

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    Question

    Thank you for your replacement AB6608 which our customer has received in the meantime. Unfortunately the problem (many spots on the blot) does also appear with this new vial (which is of the same lot 7396)! So the question is, what is the cause for this problem? Do you have any more suggestions or feedback from your lab/QC? Kind regards,

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    Answer

    Thank you for your patience. Actually we've never seen a blot like this before. We consulted with a couple of senior staffs in our company. We came out several suggestions as following: 1. Filter the whatever blocking reagent they use, may add 10% Goat serum to the blocking buffer, do the blocking for at least 1 h, R.T. 2. Do a 1st antibody negative control blot side-by-side with a complete WB 2. Since the specific bands are so strong, further dilute the antibody. The primary antibody incubation time can be shortened to 1h, R.T. There are many factors beyond our control. Please let us know if these could help. Thank you.

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    Question

    Antibody AB6608 (lot 7396) showed many small 'spots' in WB (ECL) - other antibodies used in comparable experiments worked fine. See attached complaint form for details.

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    Answer

    Thank you for your e-mail. We have solved several vial form exactly the same batch number without any problem. Has this customer performed an experiment using different primary antibodies on the same blot? Has he done a head-to-head experiment? We regret to inform you that usually having spots on the blot due to some practical problems: 1. Contamination. Customer should check that all the buffers are free from bacterial contamination and infection. Deionized water should be coming from a well maintained supply. 2. Not enough of the solution during incubation or washing. Make sure that the membrane is well emerged in the solution which is moving gently across the membrane. 3. During incubation air bubbles became trapped on the surface of the blot. Next time, gently remove all air bubbles. Should you need any more help then please do let us know.

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