存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab13989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 46.5 kDa.|
功能Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
序列相似性Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
翻译后修饰Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
- Information by UniProt
- ADRB2 antibody
- ADRB2_HUMAN antibody
- ADRB2R antibody
E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1/2000, Goat anti-IgY-HRP dilution: 1/1000. Colorimetric method for signal development. E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1/2000, Goat anti-IgY-HRP dilution: 1/1000. Colorimetric method for signal development.
This product has been referenced in:
- Koryakina Y et al. Effects of the ß-agonist, isoprenaline, on the down-regulation, functional responsiveness and trafficking of ß2-adrenergic receptors with N-terminal polymorphisms. Cell Biol Int 36:1171-83 (2012). Read more (PubMed: 22938397) »
- Valentine CD & Haggie PM Confinement of ß(1)- and ß(2)-adrenergic receptors in the plasma membrane of cardiomyocyte-like H9c2 cells is mediated by selective interactions with PDZ domain and A-kinase anchoring proteins but not caveolae. Mol Biol Cell 22:2970-82 (2011). Read more (PubMed: 21680711) »