Anti-beta 1 Adrenergic Receptor抗体(ab3442)
Key features and details
- Rabbit polyclonal to beta 1 Adrenergic Receptor
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Cat, Human, Non human primates, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-beta 1 Adrenergic Receptor抗体
参阅全部 beta 1 Adrenergic Receptor 一抗 -
描述
兔多克隆抗体to beta 1 Adrenergic Receptor -
宿主
Rabbit -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Mouse, Rat, Cat, Human, Non human primates, African green monkey
预测可用于: Rhesus monkey -
免疫原
Synthetic peptide corresponding to Mouse beta 1 Adrenergic Receptor aa 394-408.
Sequence:HGDRPRASGCLARAG
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading...
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纯度
Whole antiserum -
Primary antibody说明
Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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ICC | 1/100. Immunofluorescence staining of B1AR in mouse kidney distal tubule yields a pattern consistent with plasma membrane staining. |
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WB | 1/1000. Detects a band of approximately 50.5 kDa (predicted molecular weight: 50 kDa). |
靶标
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功能
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily. -
结构域
The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor. -
翻译后修饰
Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. -
细胞定位
Cell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression. - Information by UniProt
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数据库链接
- Entrez Gene: 493971 Cat
- Entrez Gene: 153 Human
- Entrez Gene: 11554 Mouse
- Entrez Gene: 24925 Rat
- Omim: 109630 Human
- SwissProt: Q9TST6 Cat
- SwissProt: P08588 Human
- SwissProt: P34971 Mouse
see all -
别名
- ADR B1 antibody
- ADRB 1 antibody
- ADRB1 antibody
see all
图片
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All lanes : Anti-beta 1 Adrenergic Receptor antibody (ab3442) at 1/1000 dilution
All lanes :
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Western blot analysis of ADR-Beta-1 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with ab3442 at a dilution of 1/1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1/20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed. -
ab3442 at 1/100 dilution staining beta 1 Adrenergic Receptor in untreated HeLa cells by Immunocytochemistry/ Immunofluorescence. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing ADR-Beta-1, at a dilution of 1/100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488-conjugated goat-anti-rabbit secondary antibody at a dilution of 1/400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye.
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Immunolocalization of B1AR in mouse kidney distal tubule using ab3442.
实验方案
文献 (35)
ab3442 被引用在 35 文献中.
- Dang S et al. Blockade of ß-adrenergic signaling suppresses inflammasome and alleviates cardiac fibrosis. Ann Transl Med 8:127 (2020). PubMed: 32175420
- Chen SY et al. Decreased basal and stimulated salivary parameters by histopathological lesions and secretory dysfunction of parotid and submandibular glands in rats with type 2 diabetes. Exp Ther Med 19:2707-2719 (2020). PubMed: 32256753
- Renard HF et al. Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166. Nat Commun 11:1457 (2020). PubMed: 32193381
- Sun R et al. Nanodiscs Incorporating Native ß1 Adrenergic Receptor as a Novel Approach for the Detection of Pathological Autoantibodies in Patients with Dilated Cardiomyopathy. J Appl Lab Med 4:391-403 (2019). PubMed: 31659076
- Ghosh A et al. An experimental model of Braak's pretangle proposal for the origin of Alzheimer's disease: the role of locus coeruleus in early symptom development. Alzheimers Res Ther 11:59 (2019). PubMed: 31266535