Anti-Beclin 1抗体(ab231341)
Key features and details
- Rabbit polyclonal to Beclin 1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
概述
-
产品名称
Anti-Beclin 1抗体
参阅全部 Beclin 1 一抗 -
描述
兔多克隆抗体to Beclin 1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human, Pig
预测可用于: Mouse, Rat, Chicken, Cow, Orangutan -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Human Beclin 1 aa 1-300. Expressed in E.coli. N-terminal tags.
Database link: Q14457 -
阳性对照
- WB: A431, HeLa and MCF7 cell lysate; Porcine brain tissue. Recombinant human Beclin 1 protein. IHC-P: Human cerebrum, stomach and pancreas tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab231341于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 2 µg/ml.
|
说明 |
---|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. |
靶标
-
功能
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the beclin family. -
细胞定位
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
-
数据库链接
- Entrez Gene: 527278 Cow
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q5ZKS6 Chicken
- SwissProt: Q4A1L4 Cow
- SwissProt: Q14457 Human
see all -
别名
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
图片
-
DAB stained, formalin-fixed paraffin-embedded human pancreas tissue stained for Beclin 1 using ab231341 at 30 µg/mL in immunohistochemical analysis.
-
All lanes : Anti-Beclin 1 antibody (ab231341) at 2 µg/ml
Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Porcine brain tissue -
DAB stained, formalin-fixed paraffin-embedded human stomach tissue stained for Beclin 1 using ab231341 at 30 µg/mL in immunohistochemical analysis.
-
DAB stained, formalin-fixed paraffin-embedded human cerebrum tissue stained for Beclin 1 using ab231341 at 30 µg/mL in immunohistochemical analysis.
-
Anti-Beclin 1 antibody (ab231341) at 2 µg/ml + Recombinant human Beclin 1 protein
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab231341 被引用在 3 文献中.
- Salvesen HA et al. Characterisation of autophagy disruption in the ileum of pigs infected with Lawsonia intracellularis. Vet Res Commun 46:585-592 (2022). PubMed: 34669106
- Dou X et al. Thymol Alleviates LPS-Induced Liver Inflammation and Apoptosis by Inhibiting NLRP3 Inflammasome Activation and the AMPK-mTOR-Autophagy Pathway. Nutrients 14:N/A (2022). PubMed: 35889766
- Zhao W et al. Prototheca bovis induces autophagy in bovine mammary epithelial cells via the HIF-1α and AMPKα/ULK1 pathway. Front Immunol 13:934819 (2022). PubMed: 36148236