Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF7 human breast cancer cells)
Specification
MCF7 human breast cancer cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/50
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Alexafluor 594 anti-mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/50
Additional data
Additional Notes
Antigen retrieval step was not employed. The cells used for this ICC were multiple drug resistant MCF7 cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Gerald Davies
Verified customer
提交于 Jun 03 2011