Anti-BCMA抗体- C-terminal (ab5972)
Key features and details
- Rabbit polyclonal to BCMA - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-BCMA抗体- C-terminal
参阅全部 BCMA 一抗 -
描述
兔多克隆抗体to BCMA - C-terminal -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human BCMA (C terminal).
(Peptide available asab6087) -
阳性对照
- Human spleen tissue lysate, K562 or U937 whole cell lysates.
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常规说明
Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL1/THANK/zTNF4 and April/TALL2, respectively. BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. April regulates immunological and non immunological cell growth. Three receptors, BCMA (for B cell maturation protein), TACI, and BAFFR, for BAFF were recently identified. BCMA, like TACI, binds BAFF and April. BAFF and its receptors induce B cell development and survival, activate NF-kappaB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
BCMA Antibody is affinity chromatography purified via peptide column. -
Primary antibody说明
Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL1/THANK/zTNF4 and April/TALL2, respectively. BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. April regulates immunological and non immunological cell growth. Three receptors, BCMA (for B cell maturation protein), TACI, and BAFFR, for BAFF were recently identified. BCMA, like TACI, binds BAFF and April. BAFF and its receptors induce B cell development and survival, activate NF-kappaB and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 10 µg/ml.
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| WB |
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 20 kDa.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 20 kDa. |
靶标
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功能
Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. -
组织特异性
Expressed in mature B-cells, but not in T-cells or monocytes. -
疾病相关
Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2. -
序列相似性
Contains 1 TNFR-Cys repeat. -
细胞定位
Cell membrane. Endomembrane system. Perinuclear Golgi-like structures. - Information by UniProt
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数据库链接
- Entrez Gene: 608 Human
- Omim: 109545 Human
- SwissProt: Q02223 Human
- Unigene: 2556 Human
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别名
- B cell maturation antigen antibody
- B cell maturation factor antibody
- B cell maturation protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)
ab5972 at 10µg/ml staining BCMA in human spleen cells by IHC
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Western blot - Anti-BCMA antibody - C-terminal (ab5972)Western blot analysis of BCMA in human spleen (A) tissue lysate, K562 (B), and U937 (C) cell lysates with ab5972 at 5µ g/ml. Western blot analysis of BCMA in human spleen (A) tissue lysate, K562 (B), and U937 (C) cell lysates with ab5972 at 5 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody - C-terminal (ab5972)Immunofluorescence of BCMA in Human Spleen cells using ab5972 at 10 ug/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCMA antibody - C-terminal (ab5972)Immunohistochemistry of BCMA in human spleen tissue with BCMA antibody at 5 µg/ml.
实验方案
数据表及文件
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Datasheet download
文献 (8)
ab5972 被引用在 8 文献中.
- Carrillo-Ballesteros FJ et al. B-cell activating factor receptor expression is associated with germinal center B-cell maintenance. Exp Ther Med 17:2053-2060 (2019). PubMed: 30783477
- Howard L et al. TWE-PRIL reverse signalling suppresses sympathetic axon growth and tissue innervation. Development 145:N/A (2018). PubMed: 30337376
- Dou H et al. APRIL promotes non-small cell lung cancer growth and metastasis by targeting ERK1/2 signaling. Oncotarget 8:109289-109300 (2017). PubMed: 29312608
- McWilliams TG et al. TNF superfamily member APRIL enhances midbrain dopaminergic axon growth and contributes to the nigrostriatal projection in vivo. Exp Neurol 298:97-103 (2017). ICC/IF ; Mouse . PubMed: 28911883
- Dou H et al. APRIL, BCMA and TACI proteins are abnormally expressed in non-small cell lung cancer. Oncol Lett 12:3351-3355 (2016). WB, IHC-P ; Human . PubMed: 27900003
- Osório C et al. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci 59:24-36 (2014). IHC-Fr ; Mouse . PubMed: 24444792
- Müller N et al. B Lymphocyte Stimulator (BLyS) Is Expressed in Human Adipocytes In Vivo and Is Related to Obesity but Not to Insulin Resistance. PLoS One 9:e94282 (2014). WB ; Human . PubMed: 24728308
- Brady G et al. Downregulation of RUNX1 by RUNX3 requires the RUNX3 VWRPY sequence and is essential for Epstein-Barr virus-driven B-cell proliferation. J Virol 83:6909-16 (2009). PubMed: 19403666
