概述

  • 产品名称
    Anti-Bcl-XL抗体[EPR16642]
    参阅全部 Bcl-XL 一抗
  • 描述
    兔单克隆抗体[EPR16642] to Bcl-XL
  • 宿主
    Rabbit
  • 经测试应用
    适用于: Flow Cyt, IHC-P, WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment corresponding to Human Bcl-XL aa 1 to the C-terminus.
    Database link: Q07817

  • 阳性对照
    • WB: Ramos, Jurkat, HeLa or K562 whole cell lysate. Mouse brain, heart, kidney, spleen. Rat brain, heart, kidney, spleen. C6, RAW 264.7, PC-12 or NIH/3T3 whole cell lysate. IHC-P: Human kidney, mouse colon, rat testis. ICC/IF: HeLa cells.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab178844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/20000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ICC/IF 1/1000.
IP 1/70.

靶标

  • 功能
    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • 组织特异性
    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • 序列相似性
    Belongs to the Bcl-2 family.
  • 结构域
    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • 翻译后修饰
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • 细胞定位
    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

图片

  • Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Bcl-XL/S (green) with ab178844 at a 1/1000 dilution showing cytoplasmic staining. Permeabilisation using 0.1% tritonX-100.

    Secondary ab: Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/200 dilution. Counter stain is labeling tubulin (red) with ab7291 at 1/500 dilution with secondary antibody Anti-Mouse AlexaFluor® 594 (ab150120) at 1/400 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab178844 at 1/1000 dilution, Anti-Mouse AlexaFluor® 594 (ab150120) at 1/400 dilution. -ve control 2 is ab7291 at 1/500 dilution, Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/200 dilution.

  • All lanes : Anti-Bcl-XL antibody [EPR16642] (ab178844) at 1/20000 dilution

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM /TBST

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1:2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. 

    Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.

    Negative control: Using PBS instead of primary ab, secondary ab as above.

     

  • Flow Cytometry analysis of HeLa cells labelling Bcl-XL with purified ab178844 at a dilution of 1/100 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

    Cytoplasm staining on epithelial cells of mouse colon tissue is observed.

    Negative control: Using PBS instead of primary ab, secondary ab as above.

  • Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

    Cytoplasm staining on epithelial cells of testis is observed.

    Negative control: Using PBS instead of primary ab, secondary ab as above.

     

  • Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.

    Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration:

    5% NFDM/TBST.

  • Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells is a comparison between ab178844 and a competitor’s leading rabbit polyclonal antibody.
    Labeling of Bcl-XL/S with ab178844 is conducted at 1/1000 dilution, followed by Anti-Rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/200 dilution.
    Labeling of Bcl-XL/S with competitor antibody is conducted at 1/50 dilution, followed by Anti-Rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/200 dilution.
    DAPI stains the nucleus in blue.
    -ve control 1 is ab178844 at 1/1000 dilution, Anti-Mouse Alexa Fluor® 594 (ab150120) at 1/400 dilution.
    -ve control 2 is ab7291 at 1/500 dilution, Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/200 dilution.

  • All lanes : Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
    developed using the ECL technique

    Predicted band size: 26 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

    Blocking buffer and concentration: 5% NFDM/TBST

     

     

  • All lanes : Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

    Lane 1 : Mouse brain
    Lane 2 : Mouse heart
    Lane 3 : Mouse kidney
    Lane 4 : Mouse spleen
    Lane 5 : Rat brain
    Lane 6 : Rat heart
    Lane 7 : Rat kidney
    Lane 8 : Rat spleen
    Lane 9 : C6 whole cell lysates
    Lane 10 : Raw264.7 whole cell lysates
    Lane 11 : PC-12 whole cell lysates
    Lane 12 : NIH/3T3 whole cell lysates

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 26 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

    Blocking buffer and concentration: 5% NFDM/TBST

  • This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Human kidney tissue.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with competitor rabbit polyclonal at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

  • This IHC image is a comparison between ab178844 and competitor's leading rabbit polyclonal in which both antibodies were tested on Mouse colon tissue.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasm staining on epithelial cells of mouse colon tissue is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

  • This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Rat testis tissue.
    Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasm staining on epithelial cells of testis is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

  • This immunoprecipitation image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.
    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with competitor rabbit polyclonal antibody at 1/12 dilution. Western blot was performed of the immunoprecipitate using competitor antibody at 1/100 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.
    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.

文献

This product has been referenced in:
  • Lian S  et al. Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats. Oncotarget 8:115304-115314 (2017). Read more (PubMed: 29383161) »
  • Cheng H Inhibiting CD146 by its Monoclonal Antibody AA98 Improves Radiosensitivity of Cervical Cancer Cells. Med Sci Monit 22:3328-33 (2016). WB ; Human . Read more (PubMed: 27647179) »
See all 3 Publications for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab178844.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册