Application
Western blot
Sample
Human Cell lysate - whole cell (Breast Cancer (clinical sample))
Loading amount
20 µg
Specification
Breast Cancer (clinical sample)
Treatment
patient recieved anthracycline and taxane neoadjuvant chemotherapy
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat milk
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 26 kDa
Positive control
samples were positive for BCL2L1 expression (identified by microarray analysis)
Additional data
Additional Notes
Band observed at expected molecular weight. Alpha-tubulin also present on the membrane.
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提交于 Jul 01 2011