Anti-Bcl-XL抗体[BX006 + 2H12] - N-terminal (ab201335)

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概述

  • 产品名称
    Anti-Bcl-XL抗体[BX006 + 2H12] - N-terminal
    参阅全部 Bcl-XL 一抗
  • 描述
    小鼠单克隆抗体[BX006 + 2H12] to Bcl-XL - N-terminal
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Fr, IP, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Bcl-XL aa 3-14 (N terminal) (Cysteine residue).
    Sequence:

    QSNRELVVDFLS


    Database link: Q07817

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Jurkat, K562, HL60, or HeLa Cells; Reed-Sternberg cells in Hodgkin’s lymphoma.
  • 常规说明

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

性能

应用

Our Abpromise guarantee covers the use of ab201335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 26 kDa.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

30 minutes at RT
IP Use at an assay dependent concentration.

Use 0.5-1µg/500µg protein lysate
Flow Cyt Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
IHC-P Use a concentration of 0.5 - 1 µg/ml.

30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.

靶标

  • 功能
    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • 组织特异性
    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • 序列相似性
    Belongs to the Bcl-2 family.
  • 结构域
    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • 翻译后修饰
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • 细胞定位
    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

图片

  • Western blot - Anti-Bcl-XL antibody [BX006 + 2H12] - N-terminal (ab201335)
    Western blot - Anti-Bcl-XL antibody [BX006 + 2H12] - N-terminal (ab201335)
    Anti-Bcl-XL antibody [BX006 + 2H12] - N-terminal (ab201335) at 1 µg/ml + Jurkat cell lysate

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [BX006 + 2H12] - N-terminal (ab201335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [BX006 + 2H12] - N-terminal (ab201335)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin's lymphoma tissue labeling Bcl x with ab201335 at 1µg/ml.

文献

ab201335 has not yet been referenced specifically in any publications.

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