Anti-Bcl-XL抗体[BX006 + 2H12] - N-terminal (ab201335)
概述
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产品名称Anti-Bcl-XL抗体[BX006 + 2H12] - N-terminal
参阅全部 Bcl-XL 一抗 -
描述小鼠单克隆抗体[BX006 + 2H12] to Bcl-XL - N-terminal
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宿主Mouse
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经测试应用适用于: WB, ICC/IF, IHC-Fr, IP, Flow Cyt, IHC-Pmore details
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种属反应性与反应: Mouse, Rat, Human, Pig
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免疫原
Synthetic peptide corresponding to Human Bcl-XL aa 3-14 (N terminal) (Cysteine residue).
Sequence:QSNRELVVDFLS
Database link: Q07817 -
阳性对照
- Jurkat, K562, HL60, or HeLa Cells; Reed-Sternberg cells in Hodgkin’s lymphoma.
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常规说明
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading... -
纯度Protein A/G purified
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纯化说明Purified from Bioreactor Concentrate.
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克隆单克隆
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克隆编号BX006 + 2H12
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同种型IgG2a
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研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
Our Abpromise guarantee covers the use of ab201335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 26 kDa. | |
| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. | |
| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. 30 minutes at RT |
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| IP | Use at an assay dependent concentration. Use 0.5-1µg/500µg protein lysate |
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| Flow Cyt | Use 0.5-1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| IHC-P | Use a concentration of 0.5 - 1 µg/ml. 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. |
靶标
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功能Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis. -
组织特异性Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
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序列相似性Belongs to the Bcl-2 family.
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结构域The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
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翻译后修饰Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
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细胞定位Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
- Information by UniProt
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数据库链接
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- Entrez Gene: 397536 Pig
- Entrez Gene: 24888 Rat
- Omim: 600039 Human
- SwissProt: Q07817 Human
- SwissProt: Q64373 Mouse
- SwissProt: O77737 Pig
see all -
别名
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
图片
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![Western blot - Anti-Bcl-XL antibody [BX006 + 2H12] - N-terminal (ab201335)](https://www.abcam.cn/ps/products/201/ab201335/Images/ab201335-243014-ab201335b.png)
Western blot - Anti-Bcl-XL antibody [BX006 + 2H12] - N-terminal (ab201335)Anti-Bcl-XL antibody [BX006 + 2H12] - N-terminal (ab201335) at 1 µg/ml + Jurkat cell lysate
Predicted band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [BX006 + 2H12] - N-terminal (ab201335)](https://www.abcam.cn/ps/products/201/ab201335/Images/ab201335-243013-ab201335-copy.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [BX006 + 2H12] - N-terminal (ab201335)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin's lymphoma tissue labeling Bcl x with ab201335 at 1µg/ml.
实验方案
数据表及文件
文献
ab201335 has not yet been referenced specifically in any publications.
