Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Expressed in a variety of tissues.
A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Belongs to the Bcl-2 family.
BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity. The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3. The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Bcl-2 with ab196495 at 1/100 dilution.
Western blot - Anti-Bcl-2 antibody (ab196495)Singh, A. et al. Imprinting of cerebral cytochrome P450s in offsprings prenatally exposed to cypermethrin augments toxicity on rechallenge. Sci. Rep. 6, 37426; doi: 10.1038/srep37426 (2016).
All lanes : Anti-Bcl-2 antibody (ab196495)
Lane 1 : Total protein isolated from brain of rat offspring raised on control mothers Lane 2 : Total protein isolated from brain of rat offspring exposed prenatally to 5 mg/kg of cypermethrin Lane 3 : Total protein isolated from brain of rat offspring exposed post natally to 10 mg/kg of cypermethrin Lane 4 : Total protein isolated from brain of rat offspring exposed prenatally to 5 mg/kg of cypermethrin and subsequently rechallenged with cypermethrin (10 mg/kg) at adulthood