Bcl-2抗体[100/D5]| Abcam中国

产品名称

Anti-Bcl-2抗体[100/D5]
参阅全部 Bcl-2 一抗
描述

小鼠单克隆抗体[100/D5] to Bcl-2
宿主

Mouse
经测试应用

适用于: Flow Cyt, IHC-P, IHC-Fr, IP, WB, ICC/IFmore details
种属反应性

与反应: Mouse, Human
预测可用于: Cow, Dog, Chinese hamster不与反应: Rat
免疫原

Synthetic peptide corresponding to Bcl-2 aa 41-54.
Sequence:
GAAPAPGIFSSQPG-Cys

Database link: P10415

Run BLAST with Run BLAST with
阳性对照
- Follicular lymphomas or tonsil.
常规说明

This product was changed from ascites to tissue culture supernatant on 8^th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant, PBS, pH 7.3
纯度

Tissue culture supernatant
克隆

单克隆
克隆编号

100/D5
骨髓瘤

P3-NS1/1-Ag4-1
同种型

IgG1
轻链类型

kappa
研究领域

Alternative Versions
- Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)
Antibody Diluent
- Universal antibody diluent (ab79995)
Compatible Secondaries
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit (ab102918)
- APC Conjugation Kit (ab201807)
Immunohistochemistry kits
- Mouse on Mouse Polymer IHC Kit (ab127055)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
- Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
Related Products
- (R)-(-)-Deprenyl hydrochloride (Selegiline hydrochloride) (ab120604)
Unmasking reagent
- 10x Heat Mediated Antigen Retrieval Solution pH 6.0 (ab973)

Our Abpromise guarantee covers the use of ab692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Ab评论	说明
Flow Cyt		1/10. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P		Use at an assay dependent concentration.
IHC-Fr		Use at an assay dependent concentration.
IP		Use at an assay dependent concentration.
WB		1/500.
ICC/IF		Use a concentration of 5 µg/ml.

功能

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
组织特异性

Expressed in a variety of tissues.
疾病相关

A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
序列相似性

Belongs to the Bcl-2 family.
结构域

BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
翻译后修饰

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
细胞定位

Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
Target information above from: UniProt accession P10415 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 100774873 Chinese hamster
- Entrez Gene: 281020 Cow
- Entrez Gene: 403416 Dog
- Entrez Gene: 596 Human
- Entrez Gene: 12043 Mouse
- Omim: 151430 Human
- SwissProt: Q9JJV8 Chinese hamster
- SwissProt: O02718 Cow
- SwissProt: Q6R755 Dog
- SwissProt: P10415 Human
- SwissProt: P10417 Mouse
- Unigene: 150749 Human
- Unigene: 257460 Mouse
see all
别名
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
- AW986256 antibody
- B cell CLL/lymphoma 2 antibody
- B cell leukemia/lymphoma 2 antibody
- Bcl-2 antibody
- Bcl2 antibody
- BCL2_HUMAN antibody
- C430015F12Rik antibody
- D630044D05Rik antibody
- D830018M01Rik antibody
- Leukemia/lymphoma, B-cell, 2 antibody
- Oncogene B-cell leukemia 2 antibody
- PPP1R50 antibody
- Protein phosphatase 1, regulatory subunit 50 antibody
see all

Western blot - Anti-Bcl-2 antibody [100/D5] (ab692)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: BCL2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab692 observed at 26 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab692 was shown to specifically react with BCL2 when BCL2 knockout samples were used. Wild-type and BCL2 knockout samples were subjected to SDS-PAGE. Ab692 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody [100/D5] (ab692)

ab692 staining Bcl-2 in SK-N-SH cells treated with (R)-(-)-Deprenyl hydrochloride (Selegiline hydrochloride) (ab120604), by ICC/IF. Increase of Bcl-2 expression correlates with increased concentration of (R)-(-)-Deprenyl hydrochloride (Selegiline hydrochloride), as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120604 ((R)-(-)-Deprenyl hydrochloride (Selegiline hydrochloride)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab692 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A anti-mouse DyLight 488 polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [100/D5] (ab692)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Bcl-2 with ab692 at 1/100 dilution. Samples were incubated with primary antibody for 30-45 minutes at RT.
Flow Cytometry - Anti-Bcl-2 antibody [100/D5] (ab692)

Overlay histogram showing Jurkat cells stained with ab692 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab692, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

This product has been referenced in:

Yu X et al. Baicalein induces cervical cancer apoptosis through the NF-?B signaling pathway. Mol Med Rep 17:5088-5094 (2018). Read more (PubMed: 29393414) »
Ma C et al. Lentivirus-mediated MDA7/IL24 expression inhibits the proliferation of hepatocellular carcinoma cells. Mol Med Rep 17:5764-5773 (2018). Read more (PubMed: 29484443) »

See all 67 Publications for this product

发表研究结果有使用 ab692？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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1-10 of 16 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunocytochemistry

Sample

Mouse Cultured Cells (Cardiomyocytes)

Specification

Cardiomyocytes

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

提交于 Mar 13 2018

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (12)

Sample

Mouse Tissue lysate - whole (Heart)

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Oct 21 2013

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (12)

Sample

Rat Tissue lysate - whole (Heart)

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Oct 14 2013

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (12)

Sample

Human Tissue lysate - whole (Heart)

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Oct 14 2013

Question

Inquiry: I'm interested if there is a possibility to get small amount of antibodies for test purposes. If yes, what is the amount, concentration and price for that. We are interested for -Anti-CDKN1A interacting zinc finger protein 1 antibody (ab102013) -Anti-p53 antibody (ab1101) -Anti-Bcl2 antibody [100/D5] (ab692) -Anti-Bax antibody (ab7977) Thank you for answer Regards

Abcam community

Verified customer

Asked on Jul 25 2012

Answer

Thank you for contacting us. Unfortunately, as we carry over 70,000 products, it simply isn't feasible for us to keep small sample sizes of our products.

However, we are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. More information on this offer can be found from the following link:

https://www.abcam.com/collaboratordiscount

In order to qualify for this offer you need to be issued with a testing discount code prior to the purchase of the antibody to be tested. Please do therefore let us know if you would like to participate in this scheme.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Abcam Scientific Support

回复于 Jul 25 2012

Question

Hi,

I have some antibodies from you, and their datasheets say that I need to store them at 4C, for example, ab98711 and ab692.

However, most of antibodies from abcam need to be stored at –20C or –80C.

So, my question is what is the estimated shelf life of the product if I store it at 4C as its datasheet says.

I’m worried whether it is correct that they are loosing their affinity or denatured with a higher rate compared to other antibodies kept at –20C as time goes by.
And, what happens if I freeze it?

Thank you in advance.

Abcam community

Verified customer

Asked on May 22 2012

Answer

Thank you for your enquiry.

Estimated shelf life of antibodies stored at 4 degrees C is about 1 year, although frequently they last much longer.

I recommend that you follow the storage conditions as noted on the product datasheet. Freezing an antibody that is intended to only be stored at 4 degrees C may lead to denaturing of the antibody.

I hope this is helpful. Please contact me again if you have any further questions.

Abcam Scientific Support

回复于 May 22 2012

Question

Hi.

I have frozen Ab692 by a mistake. The freezer's temperature is around
-30 degrees. What should I do? Should I take it out and put it in -80 or
transfer it to a refrigerator at 4 degrees? Do you think the antibody is
still usable for IHC?

Regards,

Abcam community

Verified customer

Asked on Mar 07 2012

Answer

Thank you for your enquiry. I am sorry to hear you have some concerns regarding the storage of the antibody.

I would suggest to take the antibody out of the freezer and put it at +4oC. The antibody solution does contain some BSA, which can act as a cryoprotectant, so the antibody may be ok and I would certainly recommend it is worth trying in an experiment. However, regrettably I am sorry we would not longer be able to guarantee how well this will work.

If it has lost some activity, you could try using it at a higher concentration.

I hope this will be helpful. Please do not hesitate to let me know if you have any further questions. Good luck with the experiments.

Abcam Scientific Support

回复于 Mar 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (Glioblastoma Initiating Cells (Primary cells))

Loading amount

30 µg

Specification

Glioblastoma Initiating Cells (Primary cells)

Gel Running Conditions

Reduced Denaturing (12.5)

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Nov 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Tissue lysate - whole (human islet lysate)

Loading amount

20 µg

Specification

human islet lysate

Gel Running Conditions

Reduced Denaturing

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Mr. Hao Wu

Verified customer

提交于 Nov 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa Cells)

Loading amount

20 µg

Specification

HeLa Cells

Gel Running Conditions

Non-reduced Denaturing (10)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Aug 23 2011

1-10 of 16 Abreviews or Q&A

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

定制方案及合作关系

Anti-Bcl-2抗体[100/D5] (ab692)

概述

性能

相关产品

应用

靶标

图片

实验方案

数据表及文件

文献

This product has been referenced in:

客户评价及客户问答

Immunocytochemistry abreview for Anti-Bcl-2 antibody [100/D5]

Western blot abreview for Anti-Bcl-2 [100/D5] antibody

Western blot abreview for Anti-Bcl-2 antibody [100/D5]

Western blot abreview for Anti-Bcl-2 antibody [100/D5]

Western blot abreview for Anti-Bcl2 antibody [100/D5]

Western blot abreview for Anti-Bcl2 antibody [100/D5]

Western blot abreview for Anti-Bcl2 antibody [100/D5]