ab106084 is specific for Borrelia burgdorferi BBA64 protein. A BLAST analysis was used to suggest cross-reactivity with BBA64 from B. burgdorferi, garinii, and afzelii sources based on 100% homology with the immunizing sequence.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 35 kDa.
The BBA64 (p35 kDa) protein of the spirochete Borrelia burgdorferi is being investigated for use as an early diagnostic marker of Lyme Disease.
Borrelia may change its antigenic composition in its need for adaptation to stresses imposed by changes in conditions from cycling between its
arthropod and mammalian hosts. This group of B. burgdorferi proteins
may be induced in the tick midgut during the feeding event. The p35
protein elicits a protective immunity from wild type B. burgdorferi. It has been shown that p35 expression in B. burgdorferi is upregulated in the
stationary growth phase, and that a temperature of 34°C but not 24°C
influenced the expression.
Antigen, P35 antibody
Western blot - Anti-BBA64 antibody (ab106084)
All lanes : Anti-BBA64 antibody (ab106084) at 1/1000 dilution
Lane 1 : MBP-BBA64 fusion protein Lane 2 : MBP alone
Lysates/proteins at 0.1 µg per lane.
Secondary All lanes : Goat-anti-Rabbit HRP at 1/40000 dilution