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Cell Biology Apoptosis Intracellular Associated Proteins
使用敲除细胞株进行验证重组RabMAb

重组Anti-Bax抗体[E63] (ab32503)

  • Datasheet
  • SDS
Reviews (12) Submit a question References (882)

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Western blot - Anti-Bax antibody [E63] (ab32503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)
  • Western blot - Anti-Bax antibody [E63] (ab32503)
  • Immunoprecipitation - Anti-Bax antibody [E63] (ab32503)
  • Western blot - Anti-Bax antibody [E63] (ab32503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)
  • Western blot - Anti-Bax antibody [E63] (ab32503)
  • Western blot - Anti-Bax antibody [E63] (ab32503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)
  • Sandwich ELISA - Anti-Bax antibody [E63] (ab32503)
  • Anti-Bax antibody [E63] (ab32503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E63] to Bax
  • Suitable for: IHC-P, WB, IP, Sandwich ELISA
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Product image
Human BAX knockout HeLa cell line (ab255363)

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概述

  • 产品名称

    Anti-Bax抗体[E63]
    参阅全部 Bax 一抗
  • 描述

    兔单克隆抗体[E63] to Bax
  • 宿主

    Rabbit
  • 特异性

    Expression levels of BAX protein vary with sample type. Induction may be required if endogenous expression is low.
  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    IP
    Human
    sELISA
    Recombinant fragment
    WB
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide within Human Bax aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q07812
    (Peptide available as ab188834)

  • 阳性对照

    • WB: Recombinant Human Bax protein (Tagged) (ab85157), HeLa, HepG2, A549, C2C12 and C6 cell lysate. Wild-type Hap1 cell lysate. Rat spleen tissue lysate. IHC-P: Human lymph node and rat kidney tissues. Human lung carcinoma tissue. IP: HeLa cell lysate.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    E63
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

相关产品

  • Alternative Versions

    • HRP Anti-Bax antibody [E63] (ab205822)
    • Anti-Bax antibody [E63] - BSA and Azide free (ab216985)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human BAX knockout HeLa cell line (ab255363)
  • KO cell lysates

    • Human BAX knockout HeLa cell lysate (ab263841)
  • KO cell pellets

    • Human BAX knockout HeLa cell pellet (ab278962)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
  • Recombinant Protein

    • Recombinant Human Bax protein (ab173026)
  • Related Products

    • Anti-Cytochrome C antibody [37BA11] (ab110325)
    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • 2-Methoxyantimycin A3, Bcl-xL and Bcl-2 inhibitor (ab141523)
    • Recombinant Human Bax protein (ab173026)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Rat
Human
IP
Human
sELISA
Recombinant fragment
WB
Human
All applications
Cow
Chinese hamster
应用 Ab评论 说明
IHC-P (2)
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (8)
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP
1/100.
Sandwich ELISA
Use at an assay dependent concentration.
说明
IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP
1/100.
Sandwich ELISA
Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能

      Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
    • 组织特异性

      Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
    • 序列相似性

      Belongs to the Bcl-2 family.
    • 结构域

      Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • 细胞定位

      Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
    • Target information above from: UniProt accession Q07812 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 280730 Cow
      • Entrez Gene: 581 Human
      • Entrez Gene: 12028 Mouse
      • Entrez Gene: 24887 Rat
      • Omim: 600040 Human
      • SwissProt: O02703 Cow
      • SwissProt: Q07812 Human
      • SwissProt: Q07813 Mouse
      • SwissProt: Q63690 Rat
      • Unigene: 624291 Human
      • Unigene: 19904 Mouse
      • Unigene: 10668 Rat
      see all
    • 别名

      • Apoptosis regulator BAX antibody
      • BAX antibody
      • Bax-protein antibody
      • BAX_HUMAN antibody
      • BAXA antibody
      • Baxdelta2G9 antibody
      • Baxdelta2G9omega antibody
      • Baxdelta2omega antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein omega antibody
      • BCL2 associated X protein transcript variant delta2 antibody
      • Bcl2-L-4 antibody
      • BCL2L4 antibody
      • membrane isoform alpha antibody
      see all

    图片

    • Western blot - Anti-Bax antibody [E63] (ab32503)
      Western blot - Anti-Bax antibody [E63] (ab32503)
      All lanes : Anti-Bax antibody [E63] (ab32503) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : BAX knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Lanes 1- 2: Merged signal (red and green). Green - ab32503 observed at 21 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab32503 was shown to react with Bax in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255363 (knockout cell lysate ab263841) was used. Wild-type HeLa and BAX knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32503 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)

      Purified ab32503 staining Bax in Human lung carcinoma tissue section by immmunohistochemistry (IHC-P- Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraffin and heat mediated antigen retrieval was performed using EDTA buffer (pH 9.0). Samples were incubated with primary antibody at 1:500 dilution. A goat anti-rabbit IgG H&L (HRP) (ab97051) was used as a secondary antibody at 1:500 dilution. Cytoplasmic staining on human lung carcinoma.

    • Western blot - Anti-Bax antibody [E63] (ab32503)
      Western blot - Anti-Bax antibody [E63] (ab32503)
      All lanes : Anti-Bax antibody [E63] (ab32503)

      Lane 1 : Wild-type HAP1 cell lysate
      Lane 2 : Bax knockout HAP1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 21 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab32503 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa or ab18058, observed at 130 kDa.

      This western blot image is a comparison between ab32503 and a competitor's top cited rabbit polyclonal antibody.

    • Immunoprecipitation - Anti-Bax antibody [E63] (ab32503)
      Immunoprecipitation - Anti-Bax antibody [E63] (ab32503)

      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, 10μg
      Lane 2 (+): HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32503 in HeLa whole cell lysate

      Purified ab32503 immunoprecipitating Bax in HeLa lysates. For western blotting, the primary antibody used was purified ab32503 at 1/1000 dilution. Ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection at 1/1000 dilution. Capture antibody was used at a 1/20 dilution. Blocking and diluting buffer used was 5% NFDM/TBST.

    • Western blot - Anti-Bax antibody [E63] (ab32503)
      Western blot - Anti-Bax antibody [E63] (ab32503)
      All lanes : Anti-Bax antibody [E63] (ab32503) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 cell lysate
      Lane 2 : Bax knockout HAP1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 21 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab32503 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab32503 was shown to recognize Bax in wild-type HAP1 cells, along with additional cross-reactive bands. Wild-type and Bax knockout samples were subjected to SDS-PAGE. ab32503 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)

      IHC image of ab32503 staining Bax in rat kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32503, 1:250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-Bax antibody [E63] (ab32503)
      Western blot - Anti-Bax antibody [E63] (ab32503)

      Lane 1 = Bax protein (Tagged) (ab85157), 10 ng. Lane 2 = Extract of HeLa cells, 40 ug. Lane 3 = Extract of HepG2 cells, 40 ug. Lane 4 = Bax protein (Tagged) (ab85157), 10 ng. Lane 5 = Extract of HeLa cells, 40 ug. Lane 6 = Extract of HepG2 cells, 40 ug. SDS PAGE performed under reducing conditions (100mM DTT Sample heated at 50oC). Primary : Lanes 1-3: Anti Bax antibody (ab77566) at 1 ug/mL. Lanes 4-6: Anti Bax antibody (ab32503) at 1:2000 dilution. Secondary : Lanes 1-3: Goat anti mouse IgG(H&L)-HRP at 1:10000. Lanes 4-6: Goat anti rabbit IgG(H&L)-HRP at 1:10000. Development: ECL with 2 min exposure. Blocking: in 5% Milk + PBS for 3 hours at RT. Primary antibody: in 5% Milk + PBS overnight at 4 C. Secondary antibody: in 5% Milk + PBS for 2 hour at RT. Predicted band size : Bax 21kDa and Bax (Tagged) 49 kDa. Observed band size : Bax 21kDa and Bax (Tagged) 49 kDa.

    • Western blot - Anti-Bax antibody [E63] (ab32503)
      Western blot - Anti-Bax antibody [E63] (ab32503)
      All lanes : Anti-Bax antibody [E63] (ab32503) at 1/2000 dilution (purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell). Whole cell lysates
      Lane 2 : Hep G2 (Human hepatocellular carcinoma epithelial cell). Whole cell lysates
      Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) Whole cell lysates
      Lane 4 : A549 (Human lung carcinoma epithelial cell) Whole cell lysates
      Lane 5 : C2C12 (Mouse myoblasts myoblast) Whole cell lysates
      Lane 6 : C6 (Rat glial tumor glial cell) Whole cell lysates
      Lane 7 : Mouse brain. Whole tissue lysate
      Lanes 8 & 10 : Rat brain. Whole tissue lysate
      Lanes 9 & 11 : Rat Spleen. Whole tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 21 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?



      Blocking and Diluting buffers: 5% NFDM/TBST

      Exposure time 1~9 lanes 32 s; 10~11 lanes 3 min

      Jurkat is negative reported by PMID: 15528359. Brain is low expressed reported by PMID: 27069530.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)

      Immunohistochemical analysis of paraffin-embedded human lymph node using anti-Bax Rabbit Monoclonal Antibody (ab32503) at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Sandwich ELISA - Anti-Bax antibody [E63] (ab32503)
      Sandwich ELISA - Anti-Bax antibody [E63] (ab32503)

      Standard Curve for Bax (Analyte: Recombinant human Bax protein (tagged) ab85157) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [2D2] to Bax - BSA and Azide free (ab77566) at 0.2ug/ml and Detector Antibody Rabbit monoclonal [E63] to Bax (ab32503) at 0.5ug/ml Concentration of ab32503 may vary from lot to lot; please use this curve as guideline.

    • Anti-Bax antibody [E63] (ab32503)
      Anti-Bax antibody [E63] (ab32503)

    实验方案

    • Protocol Booklet

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (882)

    发表研究结果有使用 ab32503?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab32503 被引用在 882 文献中.

    • Tian Y  et al. Silencing of RHEB inhibits cell proliferation and promotes apoptosis in colorectal cancer cells via inhibition of the mTOR signaling pathway. J Cell Physiol 235:442-453 (2020). PubMed: 31332784
    • Li Y  et al. LncRNA-LET relieves hypoxia-induced injury in H9c2 cells through regulation of miR-138. J Cell Biochem 121:259-268 (2020). PubMed: 31222827
    • Zakhary G  et al. Modification of kynurenine pathway via inhibition of kynurenine hydroxylase attenuates surgical brain injury complications in a male rat model. J Neurosci Res 98:155-167 (2020). PubMed: 31257634
    • Sun J  et al. Silencing of long noncoding RNA HOXD-AS1 inhibits proliferation, cell cycle progression, migration and invasion of hepatocellular carcinoma cells through MEK/ERK pathway. J Cell Biochem 121:443-457 (2020). PubMed: 31231887
    • Wu Q  et al. Anti-Nogo-A antibody promotes brain function recovery after cardiopulmonary resuscitation in rats by reducing apoptosis. Mol Med Rep 21:77-88 (2020). PubMed: 31746353
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 12 Abreviews or Q&A

    Western blot abreview for Anti-Bax antibody [E63]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Treatment
    4mg/ml bevacizumab for 48hrs
    Specification
    Glioblastoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 10 2020

    Western blot abreview for Anti-Bax antibody [E63]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SKOV-3)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    SKOV-3
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Dr. Peisheng Xu

    Verified customer

    提交于 Aug 19 2019

    Western blot abreview for Anti-Bax antibody [E63]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Brain)
    Gel Running Conditions
    Non-reduced Denaturing (12% SDS-PAGE)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 11 2019

    Western blot abreview for Anti-Bax antibody [E63]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (heart fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis tris)
    Loading amount
    25 µg
    Specification
    heart fibroblasts
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 30°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 30 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bax antibody [E63]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse aorta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate solution
    Permeabilization
    Yes - sodium citrate solution
    Specification
    mouse aorta
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 09 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Bax antibody [E63]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Injured spinal cord)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton-X100
    Specification
    Injured spinal cord
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. K. A.

    Verified customer

    提交于 Sep 19 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bax antibody [E63]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer ph6.0
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 26°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 01 2016

    Western blot abreview for Anti-Bax antibody [E63]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chinese Hamster Cell lysate - whole cell (CHO cell lines)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    5 µg
    Specification
    CHO cell lines
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 11 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Bax antibody [E63]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (primary)
    Specification
    primary
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 20 2015

    Western blot abreview for Anti-Bax antibody [E63]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (13%)
    Sample
    Human Cell lysate - whole cell (HCT116 cell lysates)
    Specification
    HCT116 cell lysates
    Treatment
    DMSO control (Ctl) and 7,5 ´M CPT-11 (CPT) for 24 hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mr. Christian Marx

    Verified customer

    提交于 Nov 29 2013

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