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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-BAF53A抗体- ChIP Grade (ab3882)

  • Datasheet
Reviews (2)Q&A (3)References (10)

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Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882)
  • ChIP - Anti-BAF53A antibody - ChIP Grade (ab3882)
  • Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882)

Key features and details

  • Rabbit polyclonal to BAF53A - ChIP Grade
  • Suitable for: ChIP, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-BAF53A抗体- ChIP Grade
    参阅全部 BAF53A 一抗
  • 描述

    兔多克隆抗体to BAF53A - ChIP Grade
  • 宿主

    Rabbit
  • 特异性

    In Western blot, ab3882 detects BAF53 and Tip60-TAP in total extracts of Tip60-TAP. Following TEV elution of Tip60-TAP ab3882 only detects BAF53.
  • 经测试应用

    适用于: ChIP, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human BAF53A aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13770)

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Swi / Snf
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

Our Abpromise guarantee covers the use of ab3882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 53 kDa.

靶标

  • 相关性

    This protein is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This protein is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
  • 细胞定位

    Nuclear
  • 数据库链接

    • Entrez Gene: 86 Human
    • Entrez Gene: 56456 Mouse
    • Entrez Gene: 361925 Rat
    • Omim: 604958 Human
    • SwissProt: O96019 Human
    • SwissProt: Q9Z2N8 Mouse
    • Unigene: 435326 Human
    • Unigene: 41077 Mouse
    • Unigene: 31666 Rat
    see all
  • 别名

    • 53 kDa BRG1 associated factor A antibody
    • Actin like 6A antibody
    • Actin like protein 6A antibody
    • Actin related protein antibody
    • Actin related protein Baf53a antibody
    • actin-related protein 4 antibody
    • ACTL 6 antibody
    • ACTL 6A antibody
    • ACTL6 antibody
    • ACTL6A antibody
    • Arp4 antibody
    • ARPN BETA antibody
    • ArpNbeta antibody
    • BAF 53 antibody
    • BAF 53A antibody
    • BAF complex 53 kDa subunit antibody
    • BAF53 antibody
    • BRG1 associated factor 53A antibody
    • BRG1 associated factor antibody
    • BRG1/brm associated factor 53A antibody
    • hArpN beta antibody
    • INO80 complex subunit K antibody
    • INO80K antibody
    • MGC5382 antibody
    see all

图片

  • Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882)
    Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882)This image is courtesy of Jacques Cote, Universite Laval, Quebec, Canada
    All lanes : Anti-BAF53A antibody - ChIP Grade (ab3882) at 1/1000 dilution

    Lane 1 : TEV elution of Tip60-TAP clone 1

    Lane 2 : TEV elution of Tip60-TAP clone 2
    Lane 3 : Total extract of Tip60-TAP clone 1
    Lane 4 : Total extract of Tip60-TAP clone 2

    Performed under reducing conditions.

    Predicted band size: 47 kDa



    Total extracts from two different clones of MCF7 (Human breast adenocarcinoma cell line) cells expressing a TAP tagged version of Tip60 were partially purified over IgG sepharose resin and eluted with TEV protease.  Western blot was performed using a 1/1000 dilution of the BAF53A antibody.

    Lane 1. TEV elution of Tip60-TAP clone #1
    Lane 2. TEV elution of Tip60-TAP clone #2
    Lane 3. Total extract of Tip60-TAP clone #1
    Lane 4. Total extract of Tip60-TAP clone #2

    NB: Tip60 is detected in the total extract due to the presence of Protein A in the TAP Tag (to which the primary and secondary abs bind). The TEV protease removes the Tag in lanes 1 and 2.

  • ChIP - Anti-BAF53A antibody - ChIP Grade (ab3882)
    ChIP - Anti-BAF53A antibody - ChIP Grade (ab3882)This image is courtesy of Sylvain Daujat

    Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab3882 to BAF53A and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 8 µl of ab3882 and 2x106 cells were used in each ChIP experiment.

  • Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882)
    Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882)
    All lanes : Anti-BAF53A antibody - ChIP Grade (ab3882) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 & 3 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
    Lanes 2 & 4-5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa
    Additional bands at: 120 kDa, 18 kDa. We are unsure as to the identity of these extra bands.

实验方案

  • ChIP protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (10)

    发表研究结果有使用 ab3882?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3882 被引用在 10 文献中.

    • Lamaa A  et al. Integrated analysis of H2A.Z isoforms function reveals a complex interplay in gene regulation. Elife 9:N/A (2020). PubMed: 32109204
    • Raab JR  et al. SWI/SNF remains localized to chromatin in the presence of SCHLAP1. Nat Genet 51:26-29 (2019). PubMed: 30510238
    • Masuda Y  et al. TRIM29 regulates the assembly of DNA repair proteins into damaged chromatin. Nat Commun 6:7299 (2015). Human . PubMed: 26095369
    • Lu W  et al. Actl6a protects embryonic stem cells from differentiating into primitive endoderm. Stem Cells 33:1782-93 (2015). PubMed: 25802002
    • Singh AP & Archer TK Analysis of the SWI/SNF chromatin-remodeling complex during early heart development and BAF250a repression cardiac gene transcription during P19 cell differentiation. Nucleic Acids Res 42:2958-75 (2014). IHC-P ; Mouse . PubMed: 24335282
    • Taulli R  et al. Failure to downregulate the BAF53a subunit of the SWI/SNF chromatin remodeling complex contributes to the differentiation block in rhabdomyosarcoma. Oncogene N/A:N/A (2013). PubMed: 23728344
    • Pourfarzad F  et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep 4:589-600 (2013). WB ; Human . PubMed: 23911284
    • Nishimoto N  et al. Heterocomplex formation by Arp4 and ß-actin is involved in the integrity of the Brg1 chromatin remodeling complex. J Cell Sci 125:3870-82 (2012). PubMed: 22573825
    • Miyamoto K  et al. Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes. Genes Dev 25:946-58 (2011). IP ; Xenopus laevis . PubMed: 21536734
    • Vachtenheim J  et al. SWI/SNF chromatin remodeling complex is critical for the expression of microphthalmia-associated transcription factor in melanoma cells. Biochem Biophys Res Commun 392:454-9 (2010). WB ; Human . PubMed: 20083088

    客户评价及客户问答

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    1-5 of 5 Abreviews or Q&A

    Question

    Phone call: Customer kindly asked if they can get small sizes of antibodies before buying the whole product.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2011

    Answer

    Thank you for contacting us. As discussed on phone we unfortunately do not sell small sizes of our products. The reason is that; we have over 70,000 products in our catalogue and having the small sized sample of each would be difficult to keep. However in order to help our customers we have extended our Abpromise guarantee. As per our further conversation if you could get in touch again after selecting few products then I will certainly look into your request for further advice. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Abcam Scientific Support

    回复于 Oct 26 2011

    Question

    Customer kindly contacted us to inquire about ChIP QC testing, the image on the datasheet and protocols.

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    Abcam community

    Verified customer

    Asked on May 14 2013

    Answer

    I have had the opportunity to inquire about ChIP testing for ab3882 and the image on our datasheet in particular. This image has been provided to us by a collaborator who tested this product in ChIP for us. This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link: https://www.abcam.com/index.html?pageconfig=abpromise I have included some ChIP protocols from our site for your review. More ChIP tips can also be found at this link: www.abcam.com/index.html?pageconfig=resource&rid=310

    Read More

    Abcam Scientific Support

    回复于 May 15 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-BAF53A antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Embryonic Brain (E15))
    Specification
    Embryonic Brain (E15)
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Triton 0.5%
    Blocking step
    BSA 0.1% + Normal Goat Serum 10% as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 21 2007

    Immunocytochemistry/ Immunofluorescence abreview for Anti-BAF53A antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Jun 05 2006

    Question

    Does this antibody work in IF?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2004

    Answer

    This antibody has been tested briefly in Immunofluorescence in U20S cells (human). IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. No staining was observed. However, staining was only attempted once and this is by no means an indication that the antibody doesn't work in IF, it merely did not perform under the conditions above. Please see reviews and the datasheet for details of any further testing.

    Read More

    Abcam Scientific Support

    回复于 Sep 07 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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