重组Anti-PBRM1/BAF180抗体[BL-39-2C3] (ab243876)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-39-2C3] to PBRM1/BAF180
- Suitable for: WB, IHC-P, ICC, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-PBRM1/BAF180抗体[BL-39-2C3]
参阅全部 PBRM1/BAF180 一抗 -
描述
兔单克隆抗体[BL-39-2C3] to PBRM1/BAF180 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human PBRM1/BAF180 aa 1-300.
Database link: Q86U86 -
阳性对照
- WB: HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, and Jurkat
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常规说明
This product is sold under License from Bethyl Laboratories, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
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纯化说明
Recombinant antibody was purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
BL-39-2C3 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab243876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
1/100 - 1/500.
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IP |
Use at an assay dependent concentration.
Use 20µl/mg lysate. |
说明 |
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WB
1/1000. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
1/100 - 1/500. |
IP
Use at an assay dependent concentration. Use 20µl/mg lysate. |
靶标
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功能
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Act as a negative regulator of cell proliferation. -
组织特异性
Widely expressed. -
疾病相关
Defects in PBRM1 are a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. -
序列相似性
Contains 2 BAH domains.
Contains 6 bromo domains.
Contains 1 HMG box DNA-binding domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 55193 Human
- Entrez Gene: 66923 Mouse
- Omim: 606083 Human
- SwissProt: Q86U86 Human
- SwissProt: Q8BSQ9 Mouse
- Unigene: 189920 Human
- Unigene: 476320 Human
- Unigene: 27913 Mouse
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别名
- BAF180 antibody
- BRG1-associated factor 180 antibody
- CG11375 antibody
see all
图片
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All lanes : Anti-PBRM1/BAF180 antibody [BL-39-2C3] (ab243876) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 6 : SW620
Lane 7 : SK-MEL-28
Lane 8 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Exposure time: 10 secondsDetection: Chemiluminescence
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All lanes : Anti-PBRM1/BAF180 antibody [BL-39-2C3] (ab243876) at 1/1000 dilution
Lane 1 : Ba/F3 whole cell lysate
Lane 2 : CH-27 (Mouse lymphoma)
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Exposure time: 10 secondsDetection: Chemiluminescence
Cells prepared using NETN lysis buffer
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Formalin-fixed, paraffin-embedded human Breast tissue stained for PBRM1/BAF180 using ab243876 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. Opal™ staining and DAPI counterstain.
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Formalin-fixed, paraffin-embedded human ovarian carcinoma stained for PBRM1/BAF180 using ab243876 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
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PBRM1/BAF180 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243876 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243876 at 1/1000 dilution. Lysates prepared using NETN lysis buffer.
Detection: Chemiluminesence
Lane 1: ab243876 IP in HEK-293T whole cell lysate.
Lane 2: Contol IgG in HEK-293T whole cell lysate.
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Formaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PBRM1/BAF180 using ab243876 in ICC analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab243876 尚未被引用在任何文献中。