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RabMAb

Anti-Bad抗体[Y208] (ab32445)

Abreviews (10)Q&A (4)Specific References (33)

概述

  • 产品名称
    Anti-Bad抗体[Y208]
    参阅全部 Bad 一抗
  • 描述
    兔单克隆抗体[Y208] to Bad
  • 宿主
    Rabbit
  • 特异性
    The antibody does not cross-react with other Bcl-2 members.
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    Synthetic peptide within Human Bad (N terminal). The exact sequence is proprietary.
    (Peptide available as ab206866)

  • 阳性对照
    • Hela cell lysate, human lymphoma.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab32445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/100.

靶标

  • 功能
    Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
  • 组织特异性
    Expressed in a wide variety of tissues.
  • 序列相似性
    Belongs to the Bcl-2 family.
  • 结构域
    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 翻译后修饰
    Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
  • 细胞定位
    Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • AI325008 antibody
    • BAD antibody
    • BAD_HUMAN antibody
    • BBC 2 antibody
    • BBC2 antibody
    • BBC6 antibody
    • Bcl 2 Antagonist of Cell Death antibody
    • Bcl 2 Binding Component 6 antibody
    • BCL X / BCL 2 Binding Protein antibody
    • BCL X Binding Protein antibody
    • Bcl XL/Bcl 2 Associated Death Promoter antibody
    • Bcl-2-binding component 6 antibody
    • Bcl-2-like protein 8 antibody
    • Bcl-XL/Bcl-2-associated death promoter antibody
    • Bcl2 antagonist of cell death antibody
    • BCL2 antagonist of cell death protein antibody
    • BCL2 associated agonist of cell death antibody
    • Bcl2 Associated Death Promoter antibody
    • BCL2 binding component 6 antibody
    • BCL2 binding protein antibody
    • Bcl2 Like 8 Protein antibody
    • Bcl2-L-8 antibody
    • BCL2L8 antibody
    • Proapoptotic BH3 Only Protein antibody
    see all

图片

  • Western blot - Anti-Bad antibody [Y208] (ab32445)
    Western blot - Anti-Bad antibody [Y208] (ab32445)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: BAD knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32445 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab32445 was shown to specifically recognise BAD in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when BAD knockout cells were examined. Wild-type and BAD knockout samples were subjected to SDS-PAGE. Ab32445 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Bad antibody [Y208] (ab32445)
    Immunocytochemistry/ Immunofluorescence - Anti-Bad antibody [Y208] (ab32445)

    Immunocytochemical analysis of 2% paraformaldehyde-fixed,0.1% Triton-X100 permeabilized RAW 264.7 labeling Bad with ab32445 at 1/250dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad antibody [Y208] (ab32445)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad antibody [Y208] (ab32445)
    Immunohistochemical staining of paraffin-embedded human lymphoma using ab32445 at 1/100 dilution.
  • Western blot - Anti-Bad antibody [Y208] (ab32445)
    Western blot - Anti-Bad antibody [Y208] (ab32445)
    Anti-Bad antibody [Y208] (ab32445) at 1/5000 dilution + Hela cell lysate

    Predicted band size: 18 kDa
    Observed band size: 23 kDa (why is the actual band size different from the predicted?)

  • Flow Cytometry - Anti-Bad antibody [Y208] (ab32445)
    Flow Cytometry - Anti-Bad antibody [Y208] (ab32445)
    Overlay histogram showing MCF-7 cells stained with ab32445 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32445, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

文献

This product has been referenced in:
  • Martínez-François JR  et al. BAD and KATPchannels regulate neuron excitability and epileptiform activity. Elife 7:N/A (2018). Read more (PubMed: 29368690) »
  • You S  et al. Tunicamycin inhibits colon carcinoma growth and aggressiveness via modulation of the ERK-JNK-mediated AKT/mTOR signaling pathway. Mol Med Rep 17:4203-4212 (2018). Read more (PubMed: 29344654) »

See all 33 Publications for this product

发表研究结果有使用 ab32445？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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14 reviews or Q&A

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Votes: Highest First

Immunocytochemistry abreview for Anti-Bad antibody [Y208]

 Good
Abreviews
Application
Immunocytochemistry
Sample
Rat Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Feb 28 2018

Please send the IgG concentrations of the following lots.
ab33906 lot YH090207C -
ab33862 lot YH082410D -
ab32429 lot YG080503 -
ab40660 lot YI070802CS -
ab32445 lot YI072804DS -

ab33906 lot YH090207C - 0.3260 mg/ml

ab33862 lot YH082410D - 0.6380 mg/ml

ab32429 lot YG080503 - 0.0790 mg/ml

ab40660 lot YI070802CS - 0.1030 mg/ml

ab32445 lot YI072804DS - 0.0250 mg/ml

Immunocytochemistry abreview for Anti-Bad antibody [Y208]

 Good
Abreviews
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Read More
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Dr. Mahesh Shivananjappa

Verified customer

提交于 May 19 2012

Dear Technical service,
Which antibodies could you recommended as a 2nd step to the following products:
ab32445 and ab33862 for flow cytometry?
Awaiting your answer.
Best regards.

Thank you for contacting us.
There is a wide range of secondaries suitable to detect either ab32445 or ab33862 in Flow Cytometry. I have conducted a search through our Secondaries search tool in the Abcam Website and found more than 100 matches, d...

Read More

Ordered ab90435 by mistake and would like to return it.

Thank you for contacting Abcam.

Included in this email are the instructions for returning your item to Abcam. When you return the goods, please ensure that you follow the procedures as below:

- Return in the original Abcam box with...

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Western blot abreview for Anti-Bad antibody [Y208]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma neurospheres cells (primary))
Loading amount
30 µg
Specification
Glioblastoma neurospheres cells (primary)
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
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Abcam user community

Verified customer

提交于 Dec 06 2011

Western blot abreview for Anti-Bad antibody [Y208]

 Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
Calcium Ionophore for 1 h
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
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Dr. Mahesh Shivananjappa

Verified customer

提交于 Nov 14 2011

Good afternoon, I ordered the rabbit monoclonal antibody BAD several months ago. I now have the question if you could let me know if the antibody binds to (immunohistochemistry) the unphosphorylated or phosphorylated form of BAD? Thank you for ...

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Thank you for contacting us. I can confirm that this antibody does recongize the phosphorylated and unphosphorylated form of BAD. Indeed, there is no phosphorylation site on the immunogen sequences used for this antibody. I hope this inf...

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Flow Cytometry abreview for Anti-Bad antibody [Y208]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Mononuclear Cell Preparations)
Specification
Mononuclear Cell Preparations
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Monocytes Lymphocytes
Read More
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Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Mar 30 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-Bad antibody [Y208]

 Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PMN)
Specification
PMN
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 30min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Read More
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Username

Abcam user community

Verified customer

提交于 Dec 20 2010

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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