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Anti-BACE2抗体(ab5670)

  • Datasheet
Submit a review Submit a question References (4)

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Immunocytochemistry/ Immunofluorescence - Anti-BACE2  antibody (ab5670)
  • Western blot - Anti-BACE2  antibody (ab5670)

Key features and details

  • Rabbit polyclonal to BACE2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-BACE2 antibody [EPR23339-221] (ab270458)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-BACE2抗体
    参阅全部 BACE2 一抗
  • 描述

    兔多克隆抗体to BACE2
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human BACE2 aa 441-457.
    Sequence:

    VSEISGPFSTEDIASNC


    (Peptide available as ab5869)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Proteases
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Aspartic protease
    • BACEs
    • Neuroscience
    • Diseases

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5670于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 2 µg/ml.
ICC/IF
1/10 - 1/200.
说明
WB
Use a concentration of 2 µg/ml.
ICC/IF
1/10 - 1/200.

靶标

  • 功能

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.
  • 组织特异性

    Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate.
  • 序列相似性

    Belongs to the peptidase A1 family.
  • 翻译后修饰

    Undergoes autoproteolytic cleavage.
    Glycosylated.
  • 细胞定位

    Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface.
  • Target information above from: UniProt accession Q9Y5Z0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 25825 Human
    • Entrez Gene: 56175 Mouse
    • Omim: 605668 Human
    • SwissProt: Q9Y5Z0 Human
    • SwissProt: Q9JL18 Mouse
    • Unigene: 529408 Human
    • Unigene: 97885 Mouse
    • 别名

      • ALP 56 antibody
      • ALP56 antibody
      • Asp 1 antibody
      • ASP1 antibody
      • ASP21 antibody
      • Aspartic Like Protease 56kDa antibody
      • Aspartic-like protease 56 kDa antibody
      • Aspartyl protease 1 antibody
      • BACE 2 antibody
      • Bace2 antibody
      • BACE2_HUMAN antibody
      • Beta Secretase 2 antibody
      • Beta Site Amyloid Beta A4 Precursor Protein Cleaving Enzyme 2 antibody
      • Beta-secretase 2 antibody
      • Beta-site amyloid precursor protein cleaving enzyme 2 antibody
      • Beta-site APP cleaving enzyme 2 antibody
      • Down region aspartic protease antibody
      • Downs Syndrome Region Aspartic Protease antibody
      • DRAP antibody
      • Memapsin 1 antibody
      • Memapsin-1 antibody
      • Membrane associated aspartic protease 1 antibody
      • Membrane-associated aspartic protease 1 antibody
      • Theta-secretase antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-BACE2  antibody (ab5670)
      Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody (ab5670)

      Immunocytochemistry/Immunofluorescence analysis of BACE2 (green) showing staining in the nucleus and cytoplasm of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5670 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    • Western blot - Anti-BACE2  antibody (ab5670)
      Western blot - Anti-BACE2 antibody (ab5670)
      All lanes : Anti-BACE2 antibody (ab5670) at 1/500 dilution

      Lane 1 : Mouse spleen cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HL60 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 25 µg per lane.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (4)

    发表研究结果有使用 ab5670?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5670 被引用在 4 文献中.

    • Chen Z  et al. Cell surface GRP78 regulates BACE2 via lysosome-dependent manner to maintain mesenchymal phenotype of glioma stem cells. J Exp Clin Cancer Res 40:20 (2021). PubMed: 33413577
    • Cao K  et al. Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation. Alzheimers Res Ther 11:35 (2019). PubMed: 31010414
    • Holler CJ  et al. BACE2 expression increases in human neurodegenerative disease. Am J Pathol 180:337-50 (2012). PubMed: 22074738
    • Ahmed RR  et al. BACE1 and BACE2 enzymatic activities in Alzheimer's disease. J Neurochem 112:1045-53 (2010). WB ; Human . PubMed: 19968762

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