Anti-BACE2抗体(ab5670)
Key features and details
- Rabbit polyclonal to BACE2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-BACE2抗体
参阅全部 BACE2 一抗 -
描述
兔多克隆抗体to BACE2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human BACE2 aa 441-457.
Sequence:VSEISGPFSTEDIASNC
(Peptide available asab5869) -
常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab5670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. | |
| ICC/IF | 1/10 - 1/200. |
靶标
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功能
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. -
组织特异性
Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate. -
序列相似性
Belongs to the peptidase A1 family. -
翻译后修饰
Undergoes autoproteolytic cleavage.
Glycosylated. -
细胞定位
Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 25825 Human
- Entrez Gene: 56175 Mouse
- Entrez Gene: 288227 Rat
- Omim: 605668 Human
- SwissProt: Q9Y5Z0 Human
- SwissProt: Q9JL18 Mouse
- SwissProt: Q6IE75 Rat
- Unigene: 529408 Human
see all -
别名
- ALP 56 antibody
- ALP56 antibody
- Asp 1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody (ab5670)
Immunocytochemistry/Immunofluorescence analysis of BACE2 (green) showing staining in the nucleus and cytoplasm of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5670 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Western blot - Anti-BACE2 antibody (ab5670)All lanes : Anti-BACE2 antibody (ab5670) at 1/500 dilution
Lane 1 : Mouse spleen cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 25 µg per lane.
实验方案
数据表及文件
文献 (3)
ab5670 被引用在 3 文献中.
- Cao K et al. Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation. Alzheimers Res Ther 11:35 (2019). PubMed: 31010414
- Holler CJ et al. BACE2 expression increases in human neurodegenerative disease. Am J Pathol 180:337-50 (2012). PubMed: 22074738
- Ahmed RR et al. BACE1 and BACE2 enzymatic activities in Alzheimer's disease. J Neurochem 112:1045-53 (2010). WB ; Human . PubMed: 19968762
