Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Lamb frontal cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Lamb frontal cortex
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/8000
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Frontal cortex: as in brains of other species, a similar pattern of positivity.
Intense, occasional cell cytoplasmic positivity.
Also intense punctate membrane positivity of many neurones, including Pyramidal cells.
There is also a strong punctate positivity in small nerve fibres throughout the neuropil.
Abcam response
Thank you for your valuable feedback. ab183612 has not been previously tested in sheep tissue and your data suggests that ab183612 may not be suitable for detecting BACE1 on sheep tissue using IHC-P under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Jul 13 2016