Anti-BACE1抗体(ab2077)
Key features and details
- Rabbit polyclonal to BACE1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-BACE1抗体
参阅全部 BACE1 一抗 -
描述
兔多克隆抗体to BACE1 -
宿主
Rabbit -
Tested Applications & Species
Application Species ICC/IF MouseIHC-P MouseWB MouseHuman -
免疫原
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阳性对照
- IHC-P: Mouse brain tissue WB: A431, A549, Caco-2, Daudi, HeLa, K562, Jurkat, SK-N-SH, THP-1. Human brain tissue lysate, 3T3 lysate. ICC/IF: 3T3 cells.
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常规说明
Beta-site APP Cleaving Enzyme.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
纯化说明
BACE Antibody is Ion exchange chromatography purified. -
Primary antibody说明
Beta-site APP Cleaving Enzyme. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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ICC/IF |
Mouse
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IHC-P |
Mouse
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WB |
Mouse
Human
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应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa. We recommend overnight incubation at 4C and using 5% skim milk to block.
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ICC/IF | (2) |
Use a concentration of 10 µg/ml.
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IHC-P |
Use a concentration of 2.5 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa. We recommend overnight incubation at 4C and using 5% skim milk to block. |
ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 2.5 µg/ml. |
靶标
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功能
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
组织特异性
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
序列相似性
Belongs to the peptidase A1 family. -
结构域
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
翻译后修饰
Glycosylated. -
细胞定位
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- Unigene: 504003 Human
- Unigene: 24044 Mouse
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别名
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
图片
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All lanes :
Lane 1 : A431 (Human epidermoid carcinoma cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 4 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 6 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 7 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 8 : SK-N-SH (Human neuroblastoma cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lane 9 : THP-1 (Human monocytic leukemia cell line) whole cell lysate withAnti-BACE1 antibody (ab2077)
Lysates/proteins at 15 µg per lane.
Blocking peptides at 1 µg/ml per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Developed using the ECL technique.
Additional bands at: 65 kDa (possible glycosylated form)10% gel.
Running conditions: 130v for 2 hours.
Transfer conditions: wet, 250mA, 2 hrs (Nitrocellulose membrane).
Blocking condition: 5% non-fat dry milk in TBS, 4C, overnight.
Primary antibody incubation: Room temperature for 1 hour.
Secondary antibody incubation: Room temperature for 1 hour.
Washing conditions: 15 mL TSBT, 3 x 10 minutes.
Exposure: ECL solution
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using ab2077 at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling BACE1 with ab2077 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing both membrane and cytosol staining on NIH/3T3 cells.
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Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling BACE1 with ab2077 at 10 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4oC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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All lanes : Anti-BACE1 antibody (ab2077) at 1 µg/ml
Lane 1 : Human brain tissue lysate with absence of blocking peptide
Lane 2 : Human brain tissue
lysate with BACE1 peptide (ab7883)
Lane 3 : Mouse 3T3/NIH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Observed band size: 70 kDa why is the actual band size different from the predicted?Incubate the antibody for 1 hour at room temperature in 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody (ab2077)Image courtesy of an anonymous Abreview.
Immunocytochemiscal analysis of D54MG (human glioblastoma cell line) cells labeling BACE1 with ab2077.Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (65)
ab2077 被引用在 65 文献中.
- Ye JY et al. ß-Sitosterol treatment attenuates cognitive deficits and prevents amyloid plaque deposition in amyloid protein precursor/presenilin 1 mice. Korean J Physiol Pharmacol 24:39-46 (2020). PubMed: 31908573
- Tam JM et al. A Novel Dual Fluorochrome Near-Infrared Imaging Probe for Potential Alzheimer's Enzyme Biomarkers-BACE1 and Cathepsin D. Molecules 25:N/A (2020). PubMed: 31936569
- Chiritoiu M et al. EDEM1 Drives Misfolded Protein Degradation via ERAD and Exploits ER-Phagy as Back-Up Mechanism When ERAD Is Impaired. Int J Mol Sci 21:N/A (2020). PubMed: 32423001
- Long Z et al. Dynamic changes of autophagic flux induced by Abeta in the brain of postmortem Alzheimer's disease patients, animal models and cell models. Aging (Albany NY) 12:10912-10930 (2020). PubMed: 32535554
- Wu Q et al. SRC-1 Knockout Exerts No Effect on Amyloid ß Deposition in APP/PS1 Mice. Front Aging Neurosci 12:145 (2020). PubMed: 32625077