Anti-Azurocidin抗体(ab167336)
Key features and details
- Mouse polyclonal to Azurocidin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Azurocidin抗体
参阅全部 Azurocidin 一抗 -
描述
小鼠多克隆抗体to Azurocidin -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human Azurocidin aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Human spleen lysate; Azurocidin transfected 293T cell lysate; Human lymph node tissue; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab167336于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
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IHC-P |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G. -
序列相似性
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. -
细胞定位
Cytoplasmic granule. Cytoplasmic granules of neutrophils. - Information by UniProt
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数据库链接
- Entrez Gene: 566 Human
- Omim: 162815 Human
- SwissProt: P20160 Human
- Unigene: 72885 Human
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别名
- Azu 1 antibody
- AZU1 antibody
- Azurocidin antibody
see all
图片
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Anti-Azurocidin antibody (ab167336) at 1 µg/ml + Human spleen lysate at 50 µg
Secondary
Goat Anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 26 kDa -
All lanes : Anti-Azurocidin antibody (ab167336) at 1 µg/ml
Lane 1 : Azurocidin transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 26 kDa -
Immunofluorescent analysis of Azurocidin expression in HeLa cells using ab167336 at 10 µg/ml dilution.
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Immunohistochemical analysis of Azurocidin expression in formalin-fixed paraffin-embedded Human lymph node tissue using ab167336 at 3 µg/ml dilution
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab167336 被引用在 2 文献中.
- Naito T et al. Azurocidin is loaded into small extracellular vesicles via its N-linked glycosylation and promotes intravasation of renal cell carcinoma cells. FEBS Lett 595:2522-2532 (2021). PubMed: 34418081
- Mayer P et al. Changes in the microarchitecture of the pancreatic cancer stroma are linked to neutrophil-dependent reprogramming of stellate cells and reflected by diffusion-weighted magnetic resonance imaging. Theranostics 8:13-30 (2018). PubMed: 29290790