Application
Western blot
Sample
Human Cell lysate - whole cell (Different human tissue culture cell lines)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage)
Loading amount
40 µg
Specification
Different human tissue culture cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Milk TBS-T 5%
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Rabitt HRP Thermo
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
West Pico ECL Thermo
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 130 kDa Non-specific: 40 kDa
Positive control
1- HAP1 10 micrograms Whole cell extract (WCE)
2- HAP1 40 micrograms Whole cell extract (WCE)
3- U2OS 10 micrograms Whole cell extract (WCE)
4- U2OS 40 micrograms Whole cell extract (WCE)
5- HELA 10 micrograms Whole cell extract (WCE)
6- HELA 40 micrograms Whole cell extract (WCE)
7- HEK293 10 micrograms Whole cell extract (WCE)
8- HEK293 40 micrograms Whole cell extract (WCE)
Additional data
Additional Notes
Seems specific but signal is weak...Maybe CEP131/AZI1 protein levels in cells is low?
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 18 2017