Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [7E10] to Axl
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
参阅全部 Axl 一抗
描述小鼠单克隆抗体[7E10] to Axl
经测试应用适用于: Flow Cyt, WB, ICC/IFmore details
- Flow Cyt: HeLa cells. ICC/IF: HeLa cells. WB: DU145, NCI-H1299 and HeLa whole cell lysates.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab130218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 0.974 µg/ml.|
|WB||Use a concentration of 1.947 µg/ml. Detects a band of approximately 80, 140 kDa (predicted molecular weight: 98 kDa).|
|ICC/IF||Use a concentration of 7.788 µg/ml.|
功能May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
组织特异性Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
- Information by UniProt
- Adhesion related kinase antibody
- AI323647 antibody
- Ark antibody
All lanes : Anti-Axl antibody [7E10] (ab130218) at 1.947 µg/ml
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : DU145 (human prostate carcinoma epithelial cell), whole cell lysate
Lane 3 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate
Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 98 kDa
Observed band size: 140,80 kDa why is the actual band size different from the predicted?
Exposure time: 180 seconds
The molecular weight observed was consistant with the literature (PMID: 19541935).
Negative control: Jurkat (PMID: 28423548).
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% methanol-fixed, 0.1% Triton X-100 permeabilized NCI-H1299 cells labeling Axl with ab130218 at 7.788 µg/mL, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on NCI-H1299 cell line.
Negative control: Jurkat (PMID:28423548).
The nuclear counterstain is DAPI (blue). Tubulin is detected with ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab130218 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: ab179513 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.
Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte, Left panel) / HeLa (Human cervix adenocarcinoma epithelial cell, Right panel) labeling Axl using Anti-Axl antibody [7E10] (ab130218) at 0.974 µg/mL. Mouse monoclonal IgG isotype control (Black). The unlabeled control is cells without incubation with primary antibody or secondary antibody (Blue).
Negative control: Jurkat.
Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.