Anti-Axin 2抗体(ab133223)
概述
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产品名称Anti-Axin 2抗体
参阅全部 Axin 2 一抗 -
描述兔多克隆抗体to Axin 2
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宿主Rabbit
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特异性At least four isoforms of Axin 2 are known to exist; this antibody will recognize all four.
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经测试应用适用于: WB, IHC-Pmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide, corresponding to amino acids from C terminal region of Human Axin 2.
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阳性对照
- Human liver and uterus tissues.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
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存储溶液Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab133223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
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组织特异性Expressed in brain and lymphoblast.
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疾病相关Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types. -
序列相似性Contains 1 DIX domain.
Contains 1 RGS domain. -
结构域The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
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翻译后修饰Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. -
细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 8313 Human
- Omim: 604025 Human
- SwissProt: Q9Y2T1 Human
- Unigene: 156527 Human
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别名
- Axil antibody
- Axin like protein antibody
- Axin-2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab133223)Immunohistochemical analysis of formalin fixed, paraffin embedded Human uterus tissue labelling Axin 2 with ab133223 at 5 ug/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab133223)Immunohistochemical analysis of formalin fixed, paraffin embedded Human liver tissue labelling Axin 2 with ab133223 at 5 ug/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
数据表及文件
文献
ab133223 has not yet been referenced specifically in any publications.
