Application
Western blot
Sample
Mouse Cell lysate - nuclear (N2a mouse neuroblastoma cells)
Loading amount
150000 cells
Specification
N2a mouse neuroblastoma cells
Treatment
lysed in radioimmunoprecipitation (RIPA) buffer (150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 50 mM Tris-HCl, pH 7.5, 2 mM EDTA) supplemented with protease inhibitor cocktail for 20 min. on ice
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% skim milk and 0.3% Tween-20 in PBS
Secondary antibody
Name
Dilution
1/10000
Detection
Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 140 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Laura Malki Feldman
Verified customer
提交于 Nov 03 2011