Anti-Aurora B抗体(ab70238)
Key features and details
- Rabbit polyclonal to Aurora B
- Suitable for: WB, IP, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Aurora B抗体
参阅全部 Aurora B 一抗 -
描述
兔多克隆抗体to Aurora B -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICCmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residue 300 and the C-terminus (residue 344) of Human Aurora B (Swiss-Prot Q96GD4).
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阳性对照
- IP: Lysate from nocodazole treated HeLa cells; WB: Lysates from nocodazole treated HeLa cells; ICC: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70238于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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IP |
Use at 2-10 µg/mg of lysate.
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ICC |
1/500 - 1/2000.
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说明 |
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WB
1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
ICC
1/500 - 1/2000. |
靶标
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功能
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP. -
组织特异性
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. -
疾病相关
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. - Information by UniProt
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数据库链接
- Entrez Gene: 9212 Human
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- Unigene: 442658 Human
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别名
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
图片
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Immunoprecipitation of nocodaze-treated HeLa whole cell lysate
Lane 1: ab70238 current lot
Lane 2: ab70238 previous lot
Lane 3: Control IgG
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All lanes : Anti-Aurora B antibody (ab70238) at 0.1 µg/ml
Lane 1 : HeLa cells treated with Nocodazole at 50 µg
Lane 2 : HeLa cells mock treated at 50 µg
Lane 3 : HeLa cells treated with Nocodazole at 15 µg
Lane 4 : HeLa cells mock treated at 15 µg
Lane 5 : HeLa cells treated with Nocodazole at 5 µg
Lane 6 : HeLa cells mock treated at 5 µg
Predicted band size: 39 kDa
Exposure time: 3 minutes -
Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab70238 被引用在 3 文献中.
- Zhu Q et al. Viral-Mediated AURKB Cleavage Promotes Cell Segregation and Tumorigenesis. Cell Rep 26:3657-3671.e5 (2019). PubMed: 30917319
- Martz MK et al. Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required for the proper completion of abscission. Mol Biol Cell 24:2785-94 (2013). PubMed: 23885121
- Wang E et al. Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. J Cell Biol 194:539-49 (2011). ICC/IF ; Human . PubMed: 21844210