Anti-Aurora B抗体(ab2254)
Key features and details
- Rabbit polyclonal to Aurora B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Aurora B抗体
参阅全部 Aurora B 一抗 -
描述
兔多克隆抗体to Aurora B -
宿主
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB MouseRatHuman -
免疫原
Synthetic peptide within Human Aurora B aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab13569) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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ICC/IF |
Human
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IHC-P |
Human
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WB |
Mouse
Rat
Human
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All applications |
Hamster
Pig
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应用 | Ab评论 | 说明 |
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ICC/IF | (13) |
Use a concentration of 0.5 - 1 µg/ml.
Methanol fixation recommended. |
IHC-P | (4) |
1/200.
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WB | (14) |
1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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说明 |
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ICC/IF
Use a concentration of 0.5 - 1 µg/ml. Methanol fixation recommended. |
IHC-P
1/200. |
WB
1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
靶标
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功能
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP. -
组织特异性
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. -
疾病相关
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. - Information by UniProt
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数据库链接
- Entrez Gene: 9212 Human
- Entrez Gene: 20877 Mouse
- Entrez Gene: 396955 Pig
- Entrez Gene: 114592 Rat
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- SwissProt: O70126 Mouse
- SwissProt: Q9N0X0 Pig
see all -
别名
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
图片
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Western blot - Anti-Aurora B antibody (ab2254)Balboula and Schindler PLoS Genet. 2014 Feb 27;10(2):e1004194. doi: 10.1371/journal.pgen.1004194. eCollection 2014 Feb. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
AURKB is expressed in mouse oocytes.
(Panel D) 20 GV-intact oocytes were collected from CF1 mice and micro-injected with the indicated cRNA. Two hours after injection, the oocytes were matured to Met II in vitro (16 h). The total numbers of non-injected control oocytes (Non-inj.) are indicated in parenthesis. Total cellular lysates were probed with the indicated antibody. The panels are images of the same membrane that was stripped and re-probed. The arrows indicate the specific AURKB protein band, and the asterisk indicates a presumed degradation product of AURKB-GFP.
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Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)Balboula and Schindler PLoS Genet. 2014 Feb 27;10(2):e1004194. doi: 10.1371/journal.pgen.1004194. eCollection 2014 Feb. Fig 1.
AURKB is expressed in mouse oocytes.
(Panel A) GV-intact oocytes were collected from CF1 mice and matured in vitro for 8 h (Met I), or 16 h (Met II), prior to fixation and staining with an anti-AURKB antibody (ab2254).
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Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)Chopra et al PLoS One. 2016 Apr 20;11(4):e0153818. doi: 10.1371/journal.pone.0153818. eCollection 2016. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
HCT 116 (Human colorectal carcinoma cell line) cells were examined by immunofluorescence confocal microscopy.
Cells were treated with 500 nM AK301 for 16 hours, and then processed for Aurora B (ab2254) and β-tubulin staining (Panel B). The color key and 20 μm bars are shown. Images of representative field is shown with a 20 μm bar. End-labeled DNA is shown in red and DAPI-stained DNA is blue.
Cells cultured on coverslips were fixed with 4% paraformaldehyde at room temperature or 100% ice cold methanol at 4°C and then permeabilized with 0.5% Triton X-100 in PBS. Cells were blocked in 5% serum (in PBS) and then incubated with primary antibody (in 5% serum) on shaker for 1 h at room temperature.
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All lanes : Anti-Aurora B antibody (ab2254) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HeLa nocodozole treated cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDaBlocked with 2% BSA.
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Immunofluorescence in human cells using Rabbit polyclonal to Aurora B (red), DAPI (blue) and CREST serum (binds to centromeres)(green).
(a) HeLa cells - transition from interphase (left) through mitosis
(b) RPE-1 cells - as in (a)
(c) HeLa cells - interphase
(d) RPE-1 cells - interphase -
ab2254 stained in Hela cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2254 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody (ab2254)
IHC image of Aurora B staining in Human Lymph node Hodgkins disease formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2254, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)This image is courtesy of an Abreview from Lux Fatimathas.
ab2254 staining human A431 (epithelial) cells by ICC/IF. The sample was fixed in paraformaldehyde and permeabilized by incubation with 0.1% Triton X100. 1% BSA was used as the blocking agent prior to a 1 hour incubation with the primary antibody, diluted 1/1000 with 1% BSA made up in PBS. An Alexa Fluor® 647 conjugated Donkey anti-Rabbit IgG (H+L) antibody was used as the secondary. Blocking and antibody incubation steps were carried out at room temperature.
In this set of images, the tubulin is stained green, Aurora B in pink and DNA in blue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody (ab2254)
Rabbit polyclonal to Aurora B (ab2254) used to stain SW620 human tumour xenografts (in mouse).
The sections were microwave pretreated in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). Slides were then incubated for 1 hour with the Aurora B primary antibody diluted 1/200 in TBS, then visualised using DAB, after application of an appropriate secondary. -
All lanes : Anti-Aurora B antibody (ab2254) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Lysate
Lane 3 : Jurkat Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 37 kDa (possible isoform)
Exposure time: 150 seconds -
Anti-Aurora B antibody (ab2254) at 1/2000 dilution +
Recombinant human Aurora B protein (ab51435) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Exposure time: 30 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (154)
ab2254 被引用在 154 文献中.
- Bywater MJ et al. Reactivation of Myc transcription in the mouse heart unlocks its proliferative capacity. Nat Commun 11:1827 (2020). PubMed: 32286286
- Salmina K et al. "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres. Int J Mol Sci 21:N/A (2020). PubMed: 32316332
- Wang Y et al. Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation. Nat Commun 11:2585 (2020). PubMed: 32444791
- Moteleub HNAE et al. Predictors for hepatocellular carcinoma recurrence after microwave ablation. Clin Exp Hepatol 6:77-84 (2020). PubMed: 32728623
- Su T et al. Platelet-Inspired Nanocells for Targeted Heart Repair After Ischemia/Reperfusion Injury. Adv Funct Mater 29:N/A (2019). PubMed: 32256277