产品名称Anti-Aurora A抗体- Centrosome Marker
参阅全部 Aurora A 一抗
描述兔多克隆抗体to Aurora A - Centrosome Marker
经测试应用适用于: ICC/IF, IHC-P, WB, IPmore details
Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Serine/Threonine Kinase 15 encoded within exon 5 (LocusLink ID 8465)
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab1287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. Used at a dilution of 1/1000 for 1 hr on HeLa cells (see Abreview).|
|IHC-P||1/250 - 1/1000.|
|WB||1/4000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast, pancreas and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly after.|
|IP||Use a concentration of 2 - 10 µg/ml.|
功能Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
组织特异性Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
翻译后修饰Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
- Information by UniProt
- AIK antibody
- ARK-1 antibody
- ARK1 antibody
Anti-Aurora A antibody - Centrosome Marker (ab1287) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 20 kDa, 25 kDa, 28 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation of Human Aurora A.
35S-Met labeled whole cell lysate from cells transfected with a human Aurora A expression construct. ab1287 was used at 4 µg/ml for IP. Detection: Autoradiography.
ab1287 (1/250) detecting Aurora A in paraffin embedded Human pancreatic tumour tissue sections. Detection: DAB staining;
ab1287 at a 1/1000 dilution staining synchronised HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 1% serum prior to incubation with the primary antibody for 1 hour. A Cy5 conjugated donkey anti-rabbit antibody was used as the secondary. The image shows a DAPI panel, Aurora-A panel, and merge panel of DAPI (blue), microtubules (red) and Aurora-A (green).
This product has been referenced in:
- Yang C et al. Effects of AURKA-mediated degradation of SOD2 on mitochondrial dysfunction and cartilage homeostasis in osteoarthritis. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30811038) »
- Willems E et al. Aurora A plays a dual role in migration and survival of human glioblastoma cells according to the CXCL12 concentration. Oncogene 38:73-87 (2019). Read more (PubMed: 30082913) »