Anti-ATXN7L3抗体(ab99947)
Key features and details
- Rabbit polyclonal to ATXN7L3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ATXN7L3抗体 -
描述
兔多克隆抗体to ATXN7L3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide within Human ATXN7L3 aa 250-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001092303.1
Database link: Q14CW9 -
阳性对照
- WB: HeLa and 293T whole cell lysates, NIH/3T3 lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab99947 was affinity purified using an epitope specific to ATXN7L3 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99947于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa. |
靶标
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功能
Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. Within the complex, it is required to recruit USP22 and ENY2 into the SAGA complex. -
序列相似性
Belongs to the SGF11 family.
Contains 1 SCA7 domain.
Contains 1 SGF11-type zinc finger. -
结构域
The long N-terminal helix forms part of the 'assembly lobe' of the SAGA deubiquitination module.
The C-terminal SGF11-type zinc-finger domain together with the C-terminal catalytic domain of USP22 forms the 'catalytic lobe' of the SAGA deubiquitination module. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 525252 Cow
- Entrez Gene: 56970 Human
- Entrez Gene: 217218 Mouse
- SwissProt: Q14CW9 Human
- SwissProt: A2AWT3 Mouse
- Unigene: 512651 Human
- Unigene: 390381 Mouse
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别名
- AT7L3_HUMAN antibody
- Ataxin 7 like 3 antibody
- Ataxin 7 like protein 3 antibody
see all
图片
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Anti-ATXN7L3 antibody (ab99947) at 0.4 µg/ml + NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 39 kDa
Exposure time: 3 minutes -
All lanes : Anti-ATXN7L3 antibody (ab99947) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293T whole cell lysate at 50 µg
Lane 5 : HEK293T whole cell lysate at 15 µg
Predicted band size: 39 kDa
Exposure time: 3 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab99947 被引用在 2 文献中.
- Liang J et al. Long non-coding RNA DSCAM-AS1 contributes to the tumorigenesis of cervical cancer by targeting miR-877-5p/ATXN7L3 axis. Biosci Rep 40:N/A (2020). PubMed: 31737900
- Spolverini A et al. let-7b and let-7c microRNAs promote histone H2B ubiquitylation and inhibit cell migration by targeting multiple components of the H2B deubiquitylation machinery. Oncogene 36:5819-5828 (2017). WB . PubMed: 28604753