Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
The intracellular localization of the protein is not as expected.
Abcam response
Our Scientific support team have investigated this case. Regrettably, the customer appears to have received a bad vial on this occasion. They have been provided an alternative free of charge replacement, in accordance with our Abpromise guarantee.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 20 2014