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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response ATM / ATR

Anti-ATR抗体(ab2905)

  • Datasheet
Reviews (23)Q&A (4)References (30)

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Western blot - Anti-ATR antibody (ab2905)
  • Western blot - Anti-ATR antibody (ab2905)

Key features and details

  • Rabbit polyclonal to ATR
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-ATR抗体
    参阅全部 ATR 一抗
  • 描述

    兔多克隆抗体to ATR
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Fusion protein, corresponding to amino acids 400-460 of Human ATR.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • ATM / ATR
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ATR protein (ab204140)

应用

Our Abpromise guarantee covers the use of ab2905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 305 kDa (predicted molecular weight: 309 kDa). (in lysate from UV irradiated K562 cells).

靶标

  • 功能

    Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
  • 组织特异性

    Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
  • 疾病相关

    Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
  • 序列相似性

    Belongs to the PI3/PI4-kinase family. ATM subfamily.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • 翻译后修饰

    Phosphorylated; autophosphorylates in vitro.
  • 细胞定位

    Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
  • Target information above from: UniProt accession Q13535 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 545 Human
    • Omim: 601215 Human
    • SwissProt: Q13535 Human
    • Unigene: 271791 Human
    • 别名

      • Ataxia telangiectasia and Rad3 related antibody
      • Ataxia telangiectasia and Rad3-related protein antibody
      • ATR antibody
      • ATR_HUMAN antibody
      • FCTCS antibody
      • FRAP Related Protein 1 antibody
      • FRAP-related protein 1 antibody
      • FRP1 antibody
      • MEC1 antibody
      • MEC1 mitosis entry checkpoint 1 homolog antibody
      • Protein kinase ATR antibody
      • RAC3 antibody
      • Rad3 related protein antibody
      • SCKL antibody
      • SCKL1 antibody
      • Serine/threonine protein kinase ATR antibody
      • Serine/threonine-protein kinase ATR antibody
      see all

    图片

    • Western blot - Anti-ATR antibody (ab2905)
      Western blot - Anti-ATR antibody (ab2905)
      Western blot on ATR in K562 whole cell lysate using ab2905.
    • Western blot - Anti-ATR antibody (ab2905)
      Western blot - Anti-ATR antibody (ab2905)
      All lanes : Anti-ATR antibody (ab2905) at 1/1000 dilution

      Lane 1 : MCF10A breast epithelial cells, grown in normoxic conditions (20% O2). Cells were lysed with NP40.
      Lane 2 : MCF10A Breast Epithelial cells grown in hypoxic. conditions (5% O2). Cells were lysed with NP40.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti Rabbit (HRP) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 309 kDa
      Observed band size: 300 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds



      Blocking: Carried out using non fat dry milk for 1 hr at room temperature.

      This image is an edited version of an image submitted courtesy of an Abreview by Francisco Ramirez-Valle submitted on 15th November 2005. We do not have any further information relating to this image.

      See Abreview

    实验方案

    • Recommended protocol for WB with ab2905

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (30)

    发表研究结果有使用 ab2905?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2905 被引用在 30 文献中.

    • Zheng T  et al. RBMX is required for activation of ATR on repetitive DNAs to maintain genome stability. Cell Death Differ N/A:N/A (2020). PubMed: 32494026
    • Wang J  et al. Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway. J Cell Mol Med 24:3931-3947 (2020). PubMed: 32126150
    • Kim W  et al. ZFP161 regulates replication fork stability and maintenance of genomic stability by recruiting the ATR/ATRIP complex. Nat Commun 10:5304 (2019). PubMed: 31757956
    • Li J & Zheng J Theaflavins prevent cartilage degeneration via AKT/FOXO3 signaling in vitro. Mol Med Rep 19:821-830 (2019). PubMed: 30569095
    • Meng X  et al. DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma. EBioMedicine 41:185-199 (2019). PubMed: 30773478
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 27 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATR antibody - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Vinicius Kannen Cardoso

    Verified customer

    提交于 Sep 15 2016

    Western blot abreview for Anti-ATR antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (EC CELLS)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    60 µg
    Specification
    EC CELLS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 22 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ATR antibody - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
    Sample
    Human Cell (HeLa cells)
    Specification
    HeLa cells
    Permeabilization
    Yes - 0.2% Triton-X100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 20 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ATR antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Sample
    Human Cell (Colon cancer)
    Specification
    Colon cancer
    Permeabilization
    Yes - TRITON 0,1%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 14 2014

    Western blot abreview for Anti-ATR antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (3-8% gradient Tris-acetate gel)
    Sample
    Human Cell lysate - whole cell (TIG-1 primary fibroblasts)
    Specification
    TIG-1 primary fibroblasts
    Blocking step
    LI-COR­ Odyssey­ Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 16 2014

    Western blot abreview for Anti-ATR antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (3-8%)
    Sample
    Human Cell lysate - whole cell (HCT116)
    Specification
    HCT116
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 18 2013

    Western blot abreview for Anti-ATR antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (3-8%)
    Sample
    Chicken Cell lysate - whole cell (DT40)
    Specification
    DT40
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 18 2013

    Western blot abreview for Anti-ATR antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (6%)
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 02 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ATR antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Specification
    U2OS
    Fixative
    Formaldehyde
    Permeabilization
    Yes - NP40
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 31 2013

    Question

    I have a question regarding the protocol. Is it better to use a nitrocellulose membrane or a PVDF membrane for this antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 10 2012

    Answer

    That is really up to you, and what you want to do with the membrane: PVDF membranes tend to show a higher background, however, PVDF tends to be slightly more robust during handling and is easier to strip, whereas NC binds the protein stronger, but is more fragile during handling and not stable against organic solvents (if in contact by chance), and you can easier blot through.


    Read More

    Abcam Scientific Support

    回复于 Jul 10 2012

    1-10 of 27 Abreviews or Q&A

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