Anti-ATPB抗体[4.3E8.D10] - Mitochondrial Marker (ab5432)

Reviews (1)Q&A (7)References (16)
Immunocytochemistry - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
  • Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
  • Western blot - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
  • Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

Key features and details

  • Mouse monoclonal [4.3E8.D10] to ATPB - Mitochondrial Marker
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

选择批间可重复性更高的重组抗体

Product image
Anti-ATPB antibody [EPR11990] (ab170947)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-ATPB抗体[4.3E8.D10] - Mitochondrial Marker
    参阅全部 ATPB 一抗

  • 描述

    小鼠单克隆抗体[4.3E8.D10] to ATPB - Mitochondrial Marker

  • 宿主

    Mouse

  • 特异性

    Detects the beta subunit of ATP synthase (ATPB) from mouse rat and human samples. This antibody is useful as a mitochondrial marker.

  • 经测试应用

    适用于: WB, ICC/IF, IPmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Tissue, cells or virus corresponding to ATPB. Intact rat mitochondria.

  • 阳性对照

    • ICC/IF: 3T3, HEK293T, A431, rat neuronal glial cells, HeLa; IP: rat neuronal/glial cells, THP-1 cells; WB: human testis tissue lysate

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5432于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF
1/100 - 1/1000.
IP
Use a concentration of 2 - 5 µg/ml.

By immunoprecipatation, this antibody detects an 50 kDa protein representing ATP synthase (ATPB) from solubilized rat brain mitochondria.
说明
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF
1/100 - 1/1000.
IP
Use a concentration of 2 - 5 µg/ml.

By immunoprecipatation, this antibody detects an 50 kDa protein representing ATP synthase (ATPB) from solubilized rat brain mitochondria.

靶标

  • 功能

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

  • 序列相似性

    Belongs to the ATPase alpha/beta chains family.

  • 细胞定位

    Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

图片

  • Immunocytochemistry - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Immunocytochemistry - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

    Immunocytochemistry analysis of ATPB using ab5432 at 5µg/mL concentration shows staining in 4% paraformaldehyde-fixed 3T3 Cells. Secondary was Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at 1/1000 dilution. ATPB (green), and nuclei with Hoechst 33342 dye (blue) is shown. Negative control has no primary antibody

  • Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)


    ATPB was immunoprecipitated from THP-1 whole cell lysate with 5 µL ab5432.

    Lane 1: ab5432 IP in THP-1 whole cell lysate, with HRP-conjugated goat anti-mouse IgG secondary

     

    Detection: Chemiluminescence 

  • Western blot - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Western blot - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa


    Exposure time: 16 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

    Immunocytochemistry/Immunofluorescent analysis of ATPB (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with ab5432 at 1:100 overnight at 4ºC in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

    Immunofluorescent analysis of ATPB in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

    Immunofluorescent analysis of ATPB in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Immunoprecipatation of rat neuronal/glial cell extract using ab5432.
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    ICC/IF image of ab5432 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

    Immunocytochemistry/Immunofluorescence analysis of rat neuronal/glial cell culture using ab5432.

文献 (16)

发表研究结果有使用 ab5432？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5432 被引用在 16 文献中.

  • Steinberg G  et al. A lipophilic cation protects crops against fungal pathogens by multiple modes of action. Nat Commun 11:1608 (2020). PubMed: 32231209
  • Rumyantseva A  et al. DARS2 is indispensable for Purkinje cell survival and protects against cerebellar ataxia. Hum Mol Genet 29:2845-2854 (2020). PubMed: 32766765
  • Valença I  et al. Prostate Cancer Proliferation Is Affected by the Subcellular Localization of MCT2 and Accompanied by Significant Peroxisomal Alterations. Cancers (Basel) 12:N/A (2020). PubMed: 33121137
  • El-Khoury R  et al. Divergent Features of Mitochondrial Deficiencies in LGMD2A Associated With Novel Calpain-3 Mutations. J Neuropathol Exp Neurol 78:88-98 (2019). PubMed: 30500922
  • Tran L  et al. Mitochondrial ATP synthase ß-subunit production rate and ATP synthase specific activity are reduced in skeletal muscle of humans with obesity. Exp Physiol 104:126-135 (2019). PubMed: 30362197
View all Publications for this product

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Western blot abreview for Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker

 Poor
Abreviews
Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - whole (head)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
head
Blocking step
casein as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
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Ms. Zhiyin Xun

Verified customer

提交于 Aug 14 2007

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