Application
Immunocytochemistry
Sample
Human Cultured Cells (Human primary dermal fibroblasts)
Permeabilization
Yes - 0.5% TX100
Specification
Human primary dermal fibroblasts
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% TX100, 1% goat serum, 1X PBS
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
ATP synthase beta subunit mouse monoclonal antibody (ab14730, Abcam, Cambridge, UK) was used in 1:1000 dilution for specific mitochondrial staining (red). Nuclei were stained with DAPI (blue).
PubMed ID
29641545: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
提交于 Oct 24 2018