Application
Western blot
Sample
Human Cell lysate - other (human fibroblast cell and mitochondria fraction)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
human fibroblast cell and mitochondria fraction
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: LiCOR 800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
LiCOR
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 70 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 27 2017