Anti-ATP6J/ATP6V1G1抗体(ab220044)
Key features and details
- Rabbit polyclonal to ATP6J/ATP6V1G1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-ATP6J/ATP6V1G1抗体 -
描述
兔多克隆抗体to ATP6J/ATP6V1G1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human ATP6J/ATP6V1G1 aa 1 to the C-terminus.
Database link: O75348 -
阳性对照
- RT-4 and U-251 MG cell lysates; Human liver lysate; PC-3 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab220044于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 14 kDa.
|
说明 |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 14 kDa. |
靶标
-
功能
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the V-ATPase G subunit family. - Information by UniProt
-
数据库链接
- Entrez Gene: 281641 Cow
- Entrez Gene: 9550 Human
- Entrez Gene: 66290 Mouse
- Entrez Gene: 298103 Rat
- Omim: 607296 Human
- SwissProt: P79251 Cow
- SwissProt: O75348 Human
- SwissProt: Q9CR51 Mouse
see all -
别名
- ATP 6G antibody
- ATP 6J antibody
- ATP6G antibody
see all
图片
-
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized PC-3 cells labeling ATP6J/ATP6V1G1 with ab220044 at 4 μg/ml (green).
-
All lanes : Anti-ATP6J/ATP6V1G1 antibody (ab220044) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Predicted band size: 14 kDa
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab220044 被引用在 1 文献中.
- Maschalidi S et al. UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells. Nat Commun 8:1640 (2017). PubMed: 29158474