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Anti-ATP5D抗体(ab97491)

  • Datasheet
  • SDS
Submit a review Submit a question References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5D antibody (ab97491)
  • Western blot - Anti-ATP5D antibody (ab97491)
  • Western blot - Anti-ATP5D antibody (ab97491)

Key features and details

  • Rabbit polyclonal to ATP5D
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-ATP5D抗体
    参阅全部 ATP5D 一抗
  • 描述

    兔多克隆抗体to ATP5D
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Recombinant protein fragment, corresponding to a region within amino acids 1-158 of Human ATP5D (NP_001678).

  • 阳性对照

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human ATP5D protein (ab109956)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97491于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
IHC-P
1/250.
说明
WB
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
IHC-P
1/250.

靶标

  • 功能

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • 序列相似性

    Belongs to the ATPase epsilon chain family.
  • 细胞定位

    Mitochondrion. Mitochondrion inner membrane.
  • Target information above from: UniProt accession P30049 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 338081 Cow
    • Entrez Gene: 513 Human
    • Entrez Gene: 66043 Mouse
    • Entrez Gene: 245965 Rat
    • Omim: 603150 Human
    • SwissProt: P05630 Cow
    • SwissProt: P30049 Human
    • SwissProt: Q9D3D9 Mouse
    • SwissProt: P35434 Rat
    • Unigene: 418668 Human
    • Unigene: 278560 Mouse
    • Unigene: 3879 Rat
    see all
  • 别名

    • ATP synthase subunit delta, mitochondrial antibody
    • ATP synthase subunit delta, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit antibody
    • ATP5D antibody
    • ATPD_HUMAN antibody
    • F ATPase delta subunit antibody
    • F-ATPase delta subunit antibody
    • Mitochondrial ATP synthase complex delta subunit precusor antibody
    • Mitochondrial ATP synthase delta subunit antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5D antibody (ab97491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5D antibody (ab97491)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human breast cancer tissue, stained with ab97491 at 1/250.

  • Western blot - Anti-ATP5D antibody (ab97491)
    Western blot - Anti-ATP5D antibody (ab97491)
    All lanes : Anti-ATP5D antibody (ab97491) at 1/500 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 17 kDa



    12% SDS PAGE
  • Western blot - Anti-ATP5D antibody (ab97491)
    Western blot - Anti-ATP5D antibody (ab97491)
    Anti-ATP5D antibody (ab97491) at 1/1000 dilution + Mouse brain at 50 µg

    Predicted band size: 17 kDa

实验方案

  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (5)

发表研究结果有使用 ab97491?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab97491 被引用在 5 文献中.

  • Anand R  et al. MIC26 and MIC27 cooperate to regulate cardiolipin levels and the landscape of OXPHOS complexes. Life Sci Alliance 3:N/A (2020). PubMed: 32788226
  • Senapati P  et al. Hyperinsulinemia promotes aberrant histone acetylation in triple-negative breast cancer. Epigenetics Chromatin 12:44 (2019). PubMed: 31315653
  • Tan JL  et al. New High-Throughput Screening Identifies Compounds That Reduce Viability Specifically in Liver Cancer Cells That Express High Levels of SALL4 by Inhibiting Oxidative Phosphorylation. Gastroenterology 157:1615-1629.e17 (2019). PubMed: 31446059
  • Liu X  et al. Regulation of mitochondrial biogenesis in erythropoiesis by mTORC1-mediated protein translation. Nat Cell Biol 19:626-638 (2017). WB . PubMed: 28504707
  • Engelen-Lee J  et al. Proteomic profiling of the spinal cord in ALS: decreased ATP5D levels suggest synaptic dysfunction in ALS pathogenesis. Amyotroph Lateral Scler Frontotemporal Degener 18:210-220 (2017). PubMed: 27899032

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