Name: Anti-ATP1B1 antibody [M17-P5-F11]
SKU: ab2873
Rating: 4 (1 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Key features and details

Mouse monoclonal [M17-P5-F11] to ATP1B1
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Human
Isotype: IgG2a

产品名称

Anti-ATP1B1抗体[M17-P5-F11]
参阅全部 ATP1B1 一抗
描述

小鼠单克隆抗体[M17-P5-F11] to ATP1B1
宿主

Mouse
经测试应用

适用于: IHC-P, WB, ICC/IF, Flow Cytmore details
种属反应性

与反应: Mouse, Human
预测可用于: Rabbit, Guinea pig, Chimpanzee, Cynomolgus monkey
免疫原

Full length native protein (purified) corresponding to Sheep ATP1B1. Purified lamb kidney sodium/potassium ATPase beta.
表位

This antibody recognizes an epitope between amino acid residues 195-199 of sheep sodium/potassium ATPase beta 1.
常规说明

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

M17-P5-F11
同种型

IgG2a
研究领域

Compatible Secondaries
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
Recombinant Protein
- Recombinant human ATP1B1 protein (Active) (ab222961)

The Abpromise guarantee

Abpromise™承诺保证使用ab2873于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
IHC-P		1/200.
WB		1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF	(1)	1/100 - 1/1000.
Flow Cyt		1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

说明
IHC-P 1/200.
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/1000.
Flow Cyt 1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

功能

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
组织特异性

Found in most tissues.
序列相似性

Belongs to the X(+)/potassium ATPases subunit beta family.
细胞定位

Membrane.
Target information above from: UniProt accession P05026 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 481 Human
- Entrez Gene: 11931 Mouse
- Omim: 182330 Human
- SwissProt: Q9JM72 Guinea pig
- SwissProt: P05026 Human
- SwissProt: P14094 Mouse
- SwissProt: Q9TT37 Rabbit
- Unigene: 291196 Human
- Unigene: 4550 Mouse
see all
别名
- Adenosinetriphosphatase antibody
- AT1B1_HUMAN antibody
- ATP 1B antibody
- ATP1B antibody
- ATP1B1 antibody
- ATPase Na+/K+ transporting beta 1 polypeptide antibody
- Atpb 1 antibody
- ATPBS antibody
- Beta 1 subunit of Na(+) K(+) ATPase antibody
- Na K ATPase beta 1 polypeptide antibody
- Na+/K+ ATPase beta 1 subunit antibody
- Sodium/potassium dependent ATPase beta 1 subunit antibody
- Sodium/potassium transporting ATPase beta 1 chain antibody
- Sodium/potassium transporting ATPase subunit beta 1 antibody
- Sodium/potassium-dependent ATPase subunit beta-1 antibody
- Sodium/potassium-transporting ATPase subunit beta-1 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

IHC image of ab2873 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunocytochemistry/Immunofluorescence analysis of ATP1B1 shows staining in HeLa cells. ATP1B1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2873 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.
Western blot - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

All lanes : Anti-ATP1B1 antibody [M17-P5-F11] (ab2873) at 1/5000 dilution

Lane 1 : Human brain lysates
Lane 2 : Human liver lysates
Lane 3 : Human kidney lysates
Lane 4 : Mouse kidney lysates

Lysates/proteins at 25 µg per lane.

Secondary
All lanes : HRP-conjugated secondary antibody

Predicted band size: 35 kDa

Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate.
Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunocytochemistry/Immunofluorescence analysis of ATP1B1 shows staining in MCF-7 cells. ATP1B1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2873 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunocytochemistry/Immunofluorescence analysis of ATP1B1 shows staining in U251 cells. ATP1B1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2873 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.
Flow Cytometry - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Overlay histogram showing HEK293 cells stained with ab2873 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2873, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase beta ab2873 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human liver tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase beta ab2873 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [M17-P5-F11] (ab2873)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase beta ab2873 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab2873？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2873 被引用在 15 文献中.

Nepal N et al. Mechanism of Na-K-ATPase Inhibition by PGE2 in Intestinal Epithelial Cells. Cells 10:N/A (2021). PubMed: 33805551
Luo J et al. Ad- and AAV8-mediated ABCA1 gene therapy in a murine model with retinal ischemia/reperfusion injuries. Mol Ther Methods Clin Dev 20:551-558 (2021). PubMed: 33665225
Nepal N et al. Unique Regulation of Na-K-ATPase during Growth and Maturation of Intestinal Epithelial Cells. Cells 8:N/A (2019). PubMed: 31208048
Scudieri P et al. Increased expression of ATP12A proton pump in cystic fibrosis airways. JCI Insight 3:N/A (2018). PubMed: 30333310
Schiapparelli P et al. NKCC1 Regulates Migration Ability of Glioblastoma Cells by Modulation of Actin Dynamics and Interacting with Cofilin. EBioMedicine 21:94-103 (2017). ICC/IF ; Human . PubMed: 28679472

View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-3 of 3 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Bone marrow derived mast cells)

Permeabilization

Yes - 0.1% Triton-X 100

Specification

Bone marrow derived mast cells

Blocking step

BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 4°C

Fixative

Paraformaldehyde

MRS. Nicole Corder

Verified customer

提交于 Jun 25 2018

Question

Do you have a mouse IgG2a ASCITES isotype avialable to use for ab2873? Also, can you give me a little more info on the purity of the antibody? Thank you

Abcam community

Verified customer

Asked on Sep 07 2005

Answer

Thank you for your enquiry. ab2873 (Sodium/Potassium ATPase Beta) is not affinity purified but comes as a PBS-diluted ascites containing 0.05% sodium azide. Please do not hesitate to contact me should you require further assistance.

Abcam Scientific Support

回复于 Sep 12 2005

Question

For ab2871 and ab2873 - Customer would like to know if the epitope is extracellular or intracellular.

Abcam community

Verified customer

Asked on Jul 09 2004

Answer

Thank you for your patience. Purified lamb kidney beta sodium/potassium ATPase was used as the immunogen for both ab2871 and ab2873. Further characterization of the epitope region has not yet been performed. Therefore we are unable to determine the location of the epitope.

Abcam Scientific Support

回复于 Jul 16 2004

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Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

表位

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (15)

客户评价及客户问答

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta 1 Sodium Potassium ATPase antibody [M17-P5-F11]