Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
15 µg
Specification
MDCK
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: polyclonal goat anti-rabbit immunoglobulins/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL Plus
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: no kDa Non-specific: ~30 kDa
Positive control
Human kidney membrane
Additional data
Additional Notes
Blot A: ab96292
Blot B: control antibody from another company against beta Sodium Potassium ATPase
Lane 1: human kidney membrane (20µg)
Lane 2: MDCK cell lysate (15µg)
Abcam response
We agree with the researcher that this antibody seems not to recongize the dog protein. We think therefore that the antibody does not react with dog beta Sodium Potassium ATPase.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 01 2011