Anti-ATP1A3抗体[XVIF9-G10] (ab2826)

Reviews (5)Q&A (1)References (10)
Flow Cytometry - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

Key features and details

  • Mouse monoclonal [XVIF9-G10] to ATP1A3
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-ATP1A3抗体[XVIF9-G10]
    参阅全部 ATP1A3 一抗

  • 描述

    小鼠单克隆抗体[XVIF9-G10] to ATP1A3

  • 宿主

    Mouse

  • 特异性

    The immunogen used for this product shares 89% homology with ATP1A2. Cross-reactivity with this protein has not been confirmed experimentally

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Rat
    Human
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Full length native protein (purified) corresponding to Dog ATP1A3. Canine cardiac microsomes.

  • 常规说明

     This product was previously labelled as alpha 3 Sodium Potassium ATPase

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Rat
Human
IHC-P
Human
All applications
Non human primates
Amphibian
应用 Ab评论 说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF
1/50.
IHC-P
1/1000 - 1/10000.

Use at 1/1000 dilution for human, 1/10,000 dilution for mouse and rat
说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF
1/50.
IHC-P
1/1000 - 1/10000.

Use at 1/1000 dilution for human, 1/10,000 dilution for mouse and rat

靶标

  • 功能

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

  • 疾病相关

    Dystonia 12
    Alternating hemiplegia of childhood 2
    Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss

  • 序列相似性

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • AHC2 antibody
    • Alpha(III) antibody
    • AT1A3_HUMAN antibody
    • Atp1a3 antibody
    • ATPase Na+/K+ transporting alpha 3 polypeptide antibody
    • DYT 12 antibody
    • DYT12 antibody
    • MGC13276 antibody
    • Na(+)/K(+) ATPase alpha(III) subunit antibody
    • Na(+)/K(+) ATPase alpha-3 subunit antibody
    • Na+/K+ ATPase 3 antibody
    • Na+/K+ ATPase alpha 3 subunit antibody
    • RDP antibody
    • Sodium potassium ATPase alpha 3 polypeptide antibody
    • Sodium pump 3 antibody
    • Sodium pump subunit alpha-3 antibody
    • Sodium/potassium transporting ATPase alpha 3 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-3 antibody
    see all

图片

  • Flow Cytometry - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Flow Cytometry - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Overlay histogram showing SH-SY5Y cells stained with ab2826 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2826, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in U251 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in HeLa cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

文献 (10)

发表研究结果有使用 ab2826？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2826 被引用在 10 文献中.

  • Fernández ÁF  et al. Interaction between the autophagy protein Beclin 1 and Na+,K+-ATPase during starvation, exercise, and ischemia. JCI Insight 5:N/A (2020). PubMed: 31941841
  • Mota MVB  et al. ATP Synthase Subunit Beta Immunostaining is Reduced in the Sclerotic Hippocampus of Epilepsy Patients. Cell Mol Neurobiol 39:149-160 (2019). PubMed: 30539418
  • Lüders KA  et al. Maintenance of high proteolipid protein level in adult central nervous system myelin is required to preserve the integrity of myelin and axons. Glia 67:634-649 (2019). PubMed: 30637801
  • Erwig MS  et al. Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin. Elife 8:N/A (2019). PubMed: 30672734
  • Kawahata I  et al. Fatty Acid-Binding Protein 3 is Critical for a-Synuclein Uptake and MPP+-Induced Mitochondrial Dysfunction in Cultured Dopaminergic Neurons. Int J Mol Sci 20:N/A (2019). PubMed: 31661838
  • Huang H & Zhang W Bufalin induced apoptosis of bladder carcinoma cells through the inactivation of Na+K+-ATPase. Oncol Lett 16:3826-3832 (2018). PubMed: 30127995
  • Picton LD  et al. Sodium Pumps Mediate Activity-Dependent Changes in Mammalian Motor Networks. J Neurosci 37:906-921 (2017). PubMed: 28123025
  • Böhm S  et al. Peripherin-2 and Rom-1 have opposing effects on rod outer segment targeting of retinitis pigmentosa-linked peripherin-2 mutants. Sci Rep 7:2321 (2017). PubMed: 28539581
  • LaCrosse AL  et al. Contrasting the Role of xCT and GLT-1 Upregulation in the Ability of Ceftriaxone to Attenuate the Cue-Induced Reinstatement of Cocaine Seeking and Normalize AMPA Receptor Subunit Expression. J Neurosci 37:5809-5821 (2017). WB ; Rat . PubMed: 28495973
  • Datta P  et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). IF ; Mouse . PubMed: 26216965

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1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10]

 Below Average
Abreviews
abreview image
Application
Western blot
Sample
Frog Tissue lysate - whole (frog brain: forebrain, midbrain, hindbrain)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
frog brain: forebrain, midbrain, hindbrain
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

提交于 Mar 07 2019

Immunocytochemistry abreview for Anti-ATP1A3 antibody [XVIF9-G10]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (ALS patient iPSC derived Neurons)
Permeabilization
Yes - 0.1% Triton X - 5 min
Specification
ALS patient iPSC derived Neurons
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Mr. Amit Chouhan

Verified customer

提交于 Mar 02 2017

Question

Dear Abcam Team,

I am looking to perform some Immunohistochemistry experiments on rat myocardium and I was wondering whether you would recommend ab78798 or ab2826? We have not used any of these in our lab & I was looking for some guidance.

Thanks for your advice,
Yours,

Read More
Answer

Thank you for your inquiry.

As our Abpromise guarantees the species and the application stated on the datasheet, I recommend to order ab78798 if you plan to use IHC-P and ab2826 if you plan to use IHC-Fr.

Both antibodies are tested in rat and therefore covered by our guarantee. Please follow this link to review the Abpromise guarantee:

https://www.abcam.com/index.html?pageconfig=abpromise

I would like to make you aware that matching secondary antibodies can be found using our search engine:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

And we also provide many IHC tailored products:

https://www.abcam.com/index.html?pageconfig=resource&rid=12853

I hope this information is helpful. Please do not hesitate to contact me again with more detailsabout the intendedexperiments if you have any further questions.

Read More

Western blot abreview for Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (brain)
Loading amount
30 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 May 20 2010

Western blot abreview for Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Brain cortex)
Loading amount
10 µg
Specification
Brain cortex
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

提交于 Sep 09 2009

Western blot abreview for Anti-ATP1A3 antibody [XVIF9-G10]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (primary cortical neurons)
Gel Running Conditions
Reduced Denaturing
Treatment
0-35 days in culture
Specification
primary cortical neurons
Read More

Abcam user community

Verified customer

提交于 Jan 23 2008

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