Anti-ATP1A3抗体[XVIF9-G10] (ab2826)
Key features and details
- Mouse monoclonal [XVIF9-G10] to ATP1A3
- Suitable for: Flow Cyt, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-ATP1A3抗体[XVIF9-G10]
参阅全部 ATP1A3 一抗 -
描述
小鼠单克隆抗体[XVIF9-G10] to ATP1A3 -
宿主
Mouse -
特异性
The immunogen used for this product shares 89% homology with ATP1A2. Cross-reactivity with this protein has not been confirmed experimentally -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF RatHumanIHC-P Human -
免疫原
Full length native protein (purified) corresponding to Dog ATP1A3. Canine cardiac microsomes.
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常规说明
This product was previously labelled as alpha 3 Sodium Potassium ATPase
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
XVIF9-G10 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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Flow Cyt |
Human
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ICC/IF |
Rat
Human
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IHC-P |
Human
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All applications |
Non human primates
Amphibian
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应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ICC/IF |
1/50.
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IHC-P |
1/1000 - 1/10000.
Use at 1/1000 dilution for human, 1/10,000 dilution for mouse and rat |
说明 |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ICC/IF
1/50. |
IHC-P
1/1000 - 1/10000. Use at 1/1000 dilution for human, 1/10,000 dilution for mouse and rat |
靶标
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功能
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
疾病相关
Dystonia 12
Alternating hemiplegia of childhood 2
Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 478 Human
- Entrez Gene: 232975 Mouse
- Entrez Gene: 24213 Rat
- Omim: 182350 Human
- SwissProt: P13637 Human
- SwissProt: Q6PIC6 Mouse
- SwissProt: P06687 Rat
- Unigene: 515427 Human
see all -
别名
- AHC2 antibody
- Alpha(III) antibody
- AT1A3_HUMAN antibody
see all
图片
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Overlay histogram showing SH-SY5Y cells stained with ab2826 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2826, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in U251 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in HeLa cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab2826 被引用在 10 文献中.
- Fernández ÁF et al. Interaction between the autophagy protein Beclin 1 and Na+,K+-ATPase during starvation, exercise, and ischemia. JCI Insight 5:N/A (2020). PubMed: 31941841
- Mota MVB et al. ATP Synthase Subunit Beta Immunostaining is Reduced in the Sclerotic Hippocampus of Epilepsy Patients. Cell Mol Neurobiol 39:149-160 (2019). PubMed: 30539418
- Lüders KA et al. Maintenance of high proteolipid protein level in adult central nervous system myelin is required to preserve the integrity of myelin and axons. Glia 67:634-649 (2019). PubMed: 30637801
- Erwig MS et al. Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin. Elife 8:N/A (2019). PubMed: 30672734
- Kawahata I et al. Fatty Acid-Binding Protein 3 is Critical for a-Synuclein Uptake and MPP+-Induced Mitochondrial Dysfunction in Cultured Dopaminergic Neurons. Int J Mol Sci 20:N/A (2019). PubMed: 31661838
- Huang H & Zhang W Bufalin induced apoptosis of bladder carcinoma cells through the inactivation of Na+K+-ATPase. Oncol Lett 16:3826-3832 (2018). PubMed: 30127995
- Picton LD et al. Sodium Pumps Mediate Activity-Dependent Changes in Mammalian Motor Networks. J Neurosci 37:906-921 (2017). PubMed: 28123025
- Böhm S et al. Peripherin-2 and Rom-1 have opposing effects on rod outer segment targeting of retinitis pigmentosa-linked peripherin-2 mutants. Sci Rep 7:2321 (2017). PubMed: 28539581
- LaCrosse AL et al. Contrasting the Role of xCT and GLT-1 Upregulation in the Ability of Ceftriaxone to Attenuate the Cue-Induced Reinstatement of Cocaine Seeking and Normalize AMPA Receptor Subunit Expression. J Neurosci 37:5809-5821 (2017). WB ; Rat . PubMed: 28495973
- Datta P et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). IF ; Mouse . PubMed: 26216965