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Signal Transduction Metabolism Energy Metabolism
使用敲除细胞株进行验证重组RabMAb

重组Anti-ATP citrate lyase抗体[EP704Y] (ab40793)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (37)

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Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Flow Cytometry (Intracellular) - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Flow Cytometry (Intracellular) - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP704Y] to ATP citrate lyase
  • Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

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概述

  • 产品名称

    Anti-ATP citrate lyase抗体[EP704Y]
    参阅全部 ATP citrate lyase 一抗
  • 描述

    兔单克隆抗体[EP704Y] to ATP citrate lyase
  • 宿主

    Rabbit
  • 特异性

    ab40793 recognises ATP citrate lyase (ACL). The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • 经测试应用

    适用于: IHC-P, Flow Cyt (Intra), ICC/IF, WB, IPmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Common marmoset
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab207504)

  • 阳性对照

    • WB: HeLa cell lysate; NIH/3T3; rat lung; C6 lysates. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: Jurkat cell lysate; HeLa. IHC: Human clear cell carcinoma of kidney
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EP704Y
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-ATP citrate lyase antibody [EP704Y] (ab205429)
    • Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)
    • PE Anti-ATP citrate lyase antibody [EP704Y] (ab209750)
    • Anti-ATP citrate lyase antibody [EP704Y] - BSA and Azide free (ab227996)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Immunizing Peptide (Blocking)

    • ATP citrate lyase peptide (ab207504)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human ATP citrate lyase protein (His tag) (ab235007)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab40793于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For unpurified use at 1/100.

ICC/IF
1/50.
WB (2)
1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 122 kDa).Can be blocked with ATP citrate lyase peptide (ab207504).

For unpurified use at 1/1000 - 1/5000.

IP
1/20.
说明
IHC-P
Use at an assay dependent concentration.
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For unpurified use at 1/100.

ICC/IF
1/50.
WB
1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 122 kDa).Can be blocked with ATP citrate lyase peptide (ab207504).

For unpurified use at 1/1000 - 1/5000.

IP
1/20.

靶标

  • 功能

    ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
  • 序列相似性

    In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
    Contains 1 ATP-grasp domain.
  • 翻译后修饰

    ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession P53396 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 47 Human
    • Entrez Gene: 104112 Mouse
    • Entrez Gene: 24159 Rat
    • Omim: 108728 Human
    • SwissProt: P53396 Human
    • SwissProt: Q91V92 Mouse
    • SwissProt: P16638 Rat
    • Unigene: 387567 Human
    • Unigene: 282039 Mouse
    • Unigene: 29771 Rat
    see all
  • 别名

    • ACL antibody
    • Acly antibody
    • ACLY_HUMAN antibody
    • ATP citrate (pro-S) lyase antibody
    • ATP citrate lyase antibody
    • ATP citrate synthase antibody
    • ATP-citrate (pro-S-)-lyase antibody
    • ATP-citrate synthase antibody
    • ATPcitrate synthase antibody
    • ATPCL antibody
    • Citrate cleavage enzyme antibody
    • CLATP antibody
    • OTTHUMP00000164773 antibody
    see all

图片

  • Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: ATP citrate lyase knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab40793 observed at 125 kDa. Red - loading control, ab9484, observed at 37 kDa.

    Unpurified ab40793 was shown to specifically react with ATP citrate lyase in wild-type HAP1 cells as signal was lost in ATP citrate lyase knockout cells. Wild-type and ATP citrate lyase knockout samples were subjected to SDS-PAGE. ab40793 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ATP citrate lyase with purified ab40793 at 1/50. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma of kidney tissue sections labeling ATP citrate lyase with Purified ab40793 at 1:100 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Flow Cytometry (Intracellular) - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Flow Cytometry (Intracellular) - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ATP citrate lyase with purified ab40793 at 1/30 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    All lanes : Anti-ATP citrate lyase antibody [EP704Y] (ab40793) at 1/50000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 122 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

    ab40793 (purified) at 1:20 dilution (1.5μg) immunoprecipitating ATP citrate lyase in HeLa whole cell lysate.

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate,10μg
    Lane 2 (+): ab40793 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40793 in HeLa whole cell lysate. 

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    All lanes : Anti-ATP citrate lyase antibody [EP704Y] (ab40793) at 1/10000 dilution (purified)

    Lane 1 : Rat lung lysates
    Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 122 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Western blot - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Anti-ATP citrate lyase antibody [EP704Y] (ab40793) at 1/5000 dilution (unpurified) + HeLa cell lysate

    Predicted band size: 122 kDa
    Observed band size: 122 kDa

  • Flow Cytometry (Intracellular) - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Flow Cytometry (Intracellular) - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

    Overlay histogram showing HeLa cells stained withunpurified ab40793 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40793, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. 

     

     

  • Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Immunoprecipitation - Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

    Unpurified ab40793 at 1/40 immunoprecipitating ATP citrate lyase in HeLa (human cervix adenocarcinoma) whole cell lysate.

    Lane 1 (input): HeLa (human cervix adenocarcinoma) whole cell lysate 10μg

    Lane 2 (+): ab40793 + HeLa (human cervix adenocarcinoma) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40793 in HeLa (human cervix adenocarcinoma) whole cell lysate

    For western blotting, ab40793 at 1/1000 dilution and ab131366 VeriBlot for IP (HRP) was used for detection at 1/10000.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
    Anti-ATP citrate lyase antibody [EP704Y] (ab40793)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (37)

发表研究结果有使用 ab40793?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab40793 被引用在 37 文献中.

  • Wang Y  et al. Improvement of obesity-associated disorders by a small-molecule drug targeting mitochondria of adipose tissue macrophages. Nat Commun 12:102 (2021). PubMed: 33397994
  • Qiu Z  et al. Biological Behavior and Lipid Metabolism of Colon Cancer Cells are Regulated by a Combination of Sterol Regulatory Element-Binding Protein 1 and ATP Citrate Lyase. Onco Targets Ther 14:1531-1542 (2021). PubMed: 33688201
  • Verberk SGS  et al. Myeloid ATP Citrate Lyase Regulates Macrophage Inflammatory Responses In Vitro Without Altering Inflammatory Disease Outcomes. Front Immunol 12:669920 (2021). PubMed: 33981315
  • Ma X  et al. Fatostatin reverses progesterone resistance by inhibiting the SREBP1-NF-?B pathway in endometrial carcinoma. Cell Death Dis 12:544 (2021). PubMed: 34039951
  • Qiao C  et al. IGF1-mediated HOXA13 overexpression promotes colorectal cancer metastasis through upregulating ACLY and IGF1R. Cell Death Dis 12:564 (2021). PubMed: 34075028
View all Publications for this product

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-ATP citrate lyase antibody [EP704Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Marmoset (common) Tissue lysate - whole (brain)
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 05 2014

Western blot abreview for Anti-ATP citrate lyase antibody [EP704Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Head)
Loading amount
40 µg
Specification
Head
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 02 2012

Question

What's the concentration?

Read More

Abcam community

Verified customer

Asked on Nov 28 2012

Answer

Vielen Dank für Ihren Anruf.

Leider ermitteln wir für diesen Antikörper die Konzentration nicht, so dass ich Ihnen leider in diesem Fall nicht weiterhelfen kann.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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Abcam Scientific Support

回复于 Nov 28 2012

Question

How are you?

I am almost ready to submit the blots showing it worked. Whatexactlyshould I send you so I could get two new antibodies for free?

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Abcam community

Verified customer

Asked on Aug 24 2012

Answer


Please log into your Abcam account, and than open the datasheet for the antibody you would like to write a review about. On top of the datasheet, you will find five tags : Datasheet, specific references, Abreview, Scientific Support, Protocols.

You have to click (you guessed it) on the Abreview section, and just follow the Submit an Abreviewlink.

You will find a drop- down menu which is pretty self explainingfrom which you have to choose your used application, species, blocking conditions, etc. An image is always welcome as well, so feel free to upload one.

However, to inform us that this is a 100% Test-discount Review, please add one of the discount codes into the "additional information "section at the end of the review. Submit it, and voila, you are ready to order anantibody for free!

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Abcam Scientific Support

回复于 Aug 24 2012

Question

,


Thank you very much!

The first antibody is :

Anti-Histone H4 (acetyl K12) antibody (ab61238)
Which appears to also work on WB Drosophila.


The second is:

Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
Which appears to work on WB in Drosophila - I want to run more test to make sure.



What does the discount means exactly? How can I use it?


Bw

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Abcam community

Verified customer

Asked on May 08 2012

Answer

Expiration date: 08. September 2012

ab40793 -DISCOUNT CODE: xxxx


ab61238- DISCOUNT CODE: xxxx

I am very pleased to hear you accepted our offer and tested ab40793 und ab61238 in drosophila. This code will give you: 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please submit an Abreview fordrosophila for each antibody and include the aprobriatecode in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Abcam Scientific Support

回复于 May 08 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
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