Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Sample
Rat Tissue sections (Rat Lung)
Specification
Rat Lung
Permeabilization
Yes - 0.1% Tween, 0.2% Triton
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS, 2% FBS, 0.1% Tween, 0.2% Triton
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 546 Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 546
Dilution
1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Stephanie Vadasz
Verified customer
提交于 Dec 04 2014