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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response ATM / ATR

Anti-ATM抗体(ab2631)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (9)

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Western blot - Anti-ATM antibody (ab2631)

    Key features and details

    • Goat polyclonal to ATM
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-ATM抗体
      参阅全部 ATM 一抗
    • 描述

      山羊多克隆抗体to ATM
    • 宿主

      Goat
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Human
      预测可用于: Chimpanzee
    • 免疫原

      Synthetic peptide (Human) - which represents a portion of human Ataxia Telangiectasia Mutated (ATM) proteinencoded within exon 53.

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • 纯化说明

      Affinity purified using the immunising peptide immobilized on solid support.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • ATM / ATR
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • Other

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human ATM protein (ab131739)

    应用

    Our Abpromise guarantee covers the use of ab2631 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/2500 - 1/10000. Detects a band of approximately 350 kDa (predicted molecular weight: 350 kDa).

    靶标

    • 功能

      Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
    • 组织特异性

      Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
    • 疾病相关

      Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
      Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
      Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
      Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.
    • 序列相似性

      Belongs to the PI3/PI4-kinase family. ATM subfamily.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 1 PI3K/PI4K domain.
    • 结构域

      The FATC domain is required for interaction with KAT5.
    • 翻译后修饰

      Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
      Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60.
    • 细胞定位

      Nucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.
    • Target information above from: UniProt accession Q13315 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 472 Human
      • Entrez Gene: 11920 Mouse
      • Omim: 607585 Human
      • SwissProt: Q13315 Human
      • SwissProt: Q62388 Mouse
      • Unigene: 367437 Human
      • Unigene: 5088 Mouse
      • 别名

        • A-T mutated antibody
        • A-T mutated homolog antibody
        • AT mutated antibody
        • AT1 antibody
        • ATA antibody
        • Ataxia telangiectasia mutated antibody
        • Ataxia telangiectasia mutated gene antibody
        • Ataxia telangiectasia mutated homolog (human) antibody
        • Ataxia telangiectasia mutated homolog antibody
        • ATC antibody
        • ATD antibody
        • ATDC antibody
        • ATE antibody
        • ATM antibody
        • ATM serine/threonine kinase antibody
        • ATM_HUMAN antibody
        • DKFZp781A0353 antibody
        • MGC74674 antibody
        • OTTHUMP00000232981 antibody
        • Serine protein kinase ATM antibody
        • Serine-protein kinase ATM antibody
        • Serine/threonine-protein kinase ATM antibody
        • Tefu antibody
        • TEL1 antibody
        • TEL1, telomere maintenance 1, homolog antibody
        • TELO1 antibody
        • Telomere fusion protein antibody
        see all

      图片

      • Western blot - Anti-ATM antibody (ab2631)
        Western blot - Anti-ATM antibody (ab2631)

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
      • SDS
    • 文献 (9)

      发表研究结果有使用 ab2631?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2631 被引用在 9 文献中.

      • Li CG  et al. PPAR? Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis. Cell Rep 26:1333-1343.e7 (2019). PubMed: 30699358
      • Li T  et al. Loss of p53-mediated cell-cycle arrest, senescence and apoptosis promotes genomic instability and premature aging. Oncotarget 7:11838-49 (2016). WB . PubMed: 26943586
      • Turner KM  et al. Genomically amplified Akt3 activates DNA repair pathway and promotes glioma progression. Proc Natl Acad Sci U S A 112:3421-6 (2015). PubMed: 25737557
      • Osterman M  et al. Elevated DNA damage response in pancreatic cancer. Histochem Cell Biol 142:713-20 (2014). PubMed: 25002126
      • Stephan H  et al. Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis. Nucleic Acids Res 37:6028-41 (2009). WB ; Human . PubMed: 19671522
      • Liu X  et al. Acquired resistance to combination treatment with temozolomide and ABT-888 is mediated by both base excision repair and homologous recombination DNA repair pathways. Mol Cancer Res 7:1686-92 (2009). WB ; Human . PubMed: 19825992
      • De Schutter H  et al. A systematic assessment of radiation dose enhancement by 5-Aza-2'-deoxycytidine and histone deacetylase inhibitors in head-and-neck squamous cell carcinoma. Int J Radiat Oncol Biol Phys 73:904-12 (2009). WB ; Human . PubMed: 19215824
      • Turner NC  et al. A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor. EMBO J : (2008). WB ; Human . PubMed: 18388863
      • McCabe N  et al. Deficiency in the repair of DNA damage by homologous recombination and sensitivity to poly(ADP-ribose) polymerase inhibition. Cancer Res 66:8109-15 (2006). WB . PubMed: 16912188

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Question

      No western signal for mouse ATM, though the antibodies do recognize human ATM.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 20 2012

      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that the free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      回复于 Apr 20 2012

      Question

      Do you have any WB images showing molecular weight markers for these ATM antibodies? During testing, was there any evidence of additional bands observed at lower molecular weights?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 03 2012

      Answer

      Thank you for contacting us. Please find the attached image showing ab2631 at a concentration of 1 ug/mL on 50 ug of HeLa cells. I hope this helps, please let me know if you need any additional information or assistance.

      Read More

      Abcam Scientific Support

      回复于 Apr 03 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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