Western blot abreview for Anti-ATM antibody [2C1 (1A1)]

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Treatment
Doxorubicin for 3 hours
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBST
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 680

Detection

Detection method
Fluorescence detection
Bands
Specific: 250 kDa
Exposure
5 minute(s) and 0 second(s)

Dr. Andrew Cobb

Verified customer

提交于 Jul 11 2013

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