Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell line)
Gel Running Conditions
Reduced Denaturing (gel 8%)
Loading amount
20 µg
Specification
breast cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS+Tween 20 0,1%+non fat milk 1%
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Abliance anti rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
clarity ECL biorad
Negative control
shATG9A in breast cancer cell line
Exposure
30 second(s)
Bands
Specific: 94 120 140 kDa
Positive control
sh control (shC) in breast cancer cell line
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 24 2015