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Our Abpromise guarantee covers the use of ab53255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21784844|
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).|
|IHC-P||Use at an assay dependent concentration. PubMed: 20174468|
Immunohistochemical analysis of murine brain tissue, staining ATG7 with ab53255. Fixed brain sections were blocked with serum, permeabilized with 0.2% Triton X-100, and treated with primary antibody. An AlexaFluor®555-conjugated anti-rabbit IgG was used as the secondary antibody.
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