Key features and details
- Rabbit polyclonal to ATG12
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
参阅全部 ATG12 一抗
经测试应用适用于: WBmore details
种属反应性与反应: Saccharomyces cerevisiae
Recombinant full length protein corresponding to Saccharomyces cerevisiae ATG12.
- Most yeast cell lysates can be used as a positive control without induction or stimulation.
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Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
纯化说明Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
Our Abpromise guarantee covers the use of ab4752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Detects a band of approximately 22.1 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.
功能Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
序列相似性Belongs to the ATG12 family.
结构域Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
- Information by UniProt
- Apg12 antibody
- APG12-like antibody
- APG12L antibody
ab4752 generated by immunization with recombinant yeast ATG12 was tested by immunoblot against yeast lysates expressing the ATG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1:15,000 dilution of Donkey polyclonal to Goat IgG (HRP) (ab7125). Panel C shows specific reaction with ATG12 using a 1:2,000 dilution of ab4752 followed by reaction with a 1:15,000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes
ab4752 被引用在 1 文献中.
- Crews L et al. Selective Molecular Alterations in the Autophagy Pathway in Patients with Lewy Body Disease and in Models of alpha-Synucleinopathy. PLoS One 5:e9313 (2010). WB ; Human, Mouse . PubMed: 20174468