Anti-ATG12抗体(ab4752)

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Western blot - Anti-ATG12 antibody (ab4752)

    Key features and details

    • Rabbit polyclonal to ATG12
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG

    概述

    • 产品名称

      Anti-ATG12抗体
      参阅全部 ATG12 一抗

    • 描述

      兔多克隆抗体to ATG12

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Saccharomyces cerevisiae

    • 免疫原

      Recombinant full length protein corresponding to Saccharomyces cerevisiae ATG12.

    • 阳性对照

      • Most yeast cell lysates can be used as a positive control without induction or stimulation.

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab4752 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB
  • 应用说明
    ELISA: 1/1000 - 1/5000.
    WB: 1/2000. Detects a band of approximately 22.1 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.

    • 靶标

    • 功能

      Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.

    • 组织特异性

      Ubiquitous.

    • 序列相似性

      Belongs to the ATG12 family.

    • 结构域

      Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.

    • 细胞定位

      Cytoplasm.
    • Information by UniProt

    • 别名

      • Apg12 antibody
      • APG12-like antibody
      • APG12L antibody
      • ATG12 antibody
      • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
      • ATG12 autophagy related 12 homolog antibody
      • ATG12_HUMAN antibody
      • Autophagy 12 antibody
      • Autophagy-related protein 12 antibody
      • FBR93 antibody
      • HAPG12 antibody
      • Ubiquitin-like protein ATG12 antibody
      see all

    图片

    • Western blot - Anti-ATG12 antibody (ab4752)
      Western blot - Anti-ATG12 antibody (ab4752)

      ab4752 generated by immunization with recombinant yeast ATG12 was tested by immunoblot against yeast lysates expressing the ATG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.   Panel A shows total protein staining using ponceau.  Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1:15,000 dilution of Donkey polyclonal to Goat IgG (HRP) (ab7125).  Panel C shows specific reaction with ATG12 using a 1:2,000 dilution of ab4752 followed by reaction with a 1:15,000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090).  All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes

    文献 (1)

    发表研究结果有使用 ab4752？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4752 被引用在 1 文献中.

    • Crews L  et al. Selective Molecular Alterations in the Autophagy Pathway in Patients with Lewy Body Disease and in Models of alpha-Synucleinopathy. PLoS One 5:e9313 (2010). WB ; Human, Mouse . PubMed: 20174468

    客户评价及客户问答

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    Question

    I am interested in your rabbit antibody to Apg12 (ab4752). On the data sheet, it says that it has not been tested in other species. Is there an update on this? Do you know if it will react with human Apg12? Thank you.

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    Answer

    Sorry but there has been no further information on this and we do not know if it will cross react with human.

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