Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Dorsal Root Ganglion)
Specification
Dorsal Root Ganglion
Other product details
Incubation time
72 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS Triton X-100 (0.3%) Na azide (0.1%)
Dilution
1/750
Secondary antibody
Dilution
1/800
Name
Non-Abcam antibody was used: Jackson Immune Research: Cy™2 AffiniPure Donkey An
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Additional data
Additional Notes
This antibody was used to label injured DRG neurons following a peripheral nerve injury. Ipsi- and contra- lateral DRGs were dissected and fixed for 24 hours at 4C in 4% Buffered Formaldehyde. several neurons in the injured DRG showed strong nuclear labelling for ATF3 antibody, whereas the contralateral DRG showed no nuclear signal, consistent with its expression in injured DRG neurons.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Steven West
Verified customer
提交于 May 24 2018