概述

  • 产品名称

  • 描述

    山羊多克隆抗体to ATF2
  • 宿主

    Goat
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human ATF2 aa 492-505 (C terminal).
    Sequence:

    C-IVMAPSSQSQPSGS


    (Peptide available as ab22789)

  • 阳性对照

    • WB: HepG2, K562 and HeLa nuclear lysates.

性能

应用

Our Abpromise guarantee covers the use of ab809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human ATF2 peptide (ab22789).

Primary incubation 1 hour at room temperature.

靶标

  • 功能

    Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • 组织特异性

    Abundant expression seen in the brain.
  • 序列相似性

    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • 翻译后修饰

    Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • 细胞定位

    Nucleus.
  • Information by UniProt
  • 数据库链接

  • 别名

    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all

图片

  • All lanes : Anti-ATF2 antibody (ab809) at 1 µg/ml

    Lane 1 : HepG2 nuclear lysate in RIPA buffer
    Lane 2 : K562 nuclear lysate in RIPA buffer
    Lane 3 : HeLa nuclear lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 54 kDa



    Detected by chemiluminescense.

实验方案

文献

This product has been referenced in:

See 1 Publication for this product

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