Anti-Aspartate Aminotransferase抗体(ab221939)
Key features and details
- Rabbit polyclonal to Aspartate Aminotransferase
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Aspartate Aminotransferase抗体
参阅全部 Aspartate Aminotransferase 一抗 -
描述
兔多克隆抗体to Aspartate Aminotransferase -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Orangutan -
免疫原
Recombinant fragment corresponding to Human Aspartate Aminotransferase aa 350 to the C-terminus.
Database link: P17174 -
阳性对照
- ICC/IF: U-2 OS cells. IHC-P: Human liver tissue. WB: Human liver and tonsil lysates; RT4 and U-251 MG cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab221939于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Plays a key role in amino acid metabolism. -
序列相似性
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 450664 Chimpanzee
- Entrez Gene: 281206 Cow
- Entrez Gene: 101865666 Cynomolgus monkey
- Entrez Gene: 100060793 Horse
- Entrez Gene: 2805 Human
- Entrez Gene: 14718 Mouse
- Entrez Gene: 100173864 Orangutan
- Entrez Gene: 396967 Pig
see all -
别名
- AATC_HUMAN antibody
- Aspartate aminotransferase 1 antibody
- Aspartate aminotransferase antibody
see all
图片
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All lanes : Anti-Aspartate Aminotransferase antibody (ab221939) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 46 kDa -
Paraffin-embedded human liver tissue stained for Aspartate Aminotransferase using ab221939 at 1/1000 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Aspartate Aminotransferase (green) using ab221939 at 4 µg/ml in ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab221939 被引用在 1 文献中.
- Wei XB et al. Exosome-Derived lncRNA NEAT1 Exacerbates Sepsis-Associated Encephalopathy by Promoting Ferroptosis Through Regulating miR-9-5p/TFRC and GOT1 Axis. Mol Neurobiol 59:1954-1969 (2022). PubMed: 35038133