Anti-ARSA/ASA抗体(ab77586)
Key features and details
- Goat polyclonal to ARSA/ASA
- Suitable for: IHC-P, WB, ICC, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ARSA/ASA抗体
参阅全部 ARSA/ASA 一抗 -
描述
山羊多克隆抗体to ARSA/ASA -
宿主
Goat -
经测试应用
适用于: IHC-P, WB, ICC, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human ARSA/ASA aa 429-440 (internal sequence).
Sequence:C-YDLSKDPGENYN
Database link: NP_000478.2 -
阳性对照
- IHC: Human cortex staining WB: Mouse testis lysates and Recombinant Human ARSA/ASA protein (ab116931) Flow Cyt (intra): HeLa cells ICC: HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab77586 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77586于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
1 hour primary incubation is recommended for this product. Approx 60-65Da band observed in Mouse and Rat Testis lysates. |
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ICC |
Use a concentration of 10 µg/ml.
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). 1 hour primary incubation is recommended for this product. Approx 60-65Da band observed in Mouse and Rat Testis lysates. |
ICC
Use a concentration of 10 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
靶标
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功能
Hydrolyzes cerebroside sulfate. -
疾病相关
Defects in ARSA are a cause of leukodystrophy metachromatic (MLD) [MIM:250100]. MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late-infantile, juvenile and adult.
Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a disorder characterized by decreased activity of all known sulfatases. MSD is due to defects in SUMF1 resulting in the lack of post-translational modification of a highly conserved cysteine into 3-oxoalanine. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. -
序列相似性
Belongs to the sulfatase family. -
翻译后修饰
The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). -
细胞定位
Lysosome. - Information by UniProt
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数据库链接
- Entrez Gene: 410 Human
- Entrez Gene: 11883 Mouse
- Omim: 607574 Human
- SwissProt: P15289 Human
- SwissProt: P50428 Mouse
- Unigene: 88251 Human
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别名
- arsA antibody
- ARSA_HUMAN antibody
- arylsulfatase A antibody
see all
图片
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Immunofluorescence analysis of HeLa cells labelling ARSA/ASA with ab77586 at 10 µg/mL. Cells were permeabilized with 0.15% Triton X-100. Alexa Fluor 488 secondary antibody (2ug/ml). Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG 10 µg/mL.
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Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling ARSA/ASA with ab77586 at 10 µg/mL. Cells were permeabilised with 0.5% Triton. Alexa Fluor 488 secondary antibody (1ug/ml). Unimmunized goat IgG was used as the isotype control (black).
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Anti-ARSA/ASA antibody (ab77586) at 0.5 µg/ml + Mouse Testis lysate (in RIPA buffer) at 35 µg
Predicted band size: 54 kDa
Observed band size: 54 kDa
Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (0)
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