Key features and details
- Rabbit polyclonal to ARPC1B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
参阅全部 ARPC1B 一抗
经测试应用适用于: WB, IPmore details
种属反应性与反应: Mouse, Human
预测可用于: Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human ARPC1B aa 272-332.
Database link: NP_005711.1
- HeLa, 293T and NIH3T3 cell lysates
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab99314 was affinity purified using an epitope specific to ARPC1B immobilized on solid support.
功能Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
序列相似性Belongs to the WD repeat ARPC1 family.
Contains 6 WD repeats.
细胞定位Cytoplasm > cytoskeleton.
- Information by UniProt
- Actin related protein 2/3 complex subunit 1B 41kDa antibody
- Actin related protein 2/3 complex subunit 1B antibody
- Actin-related protein 2/3 complex subunit 1B antibody
ab99314 at 1 µg/ml detecting ARPC1B in HeLa whole cell lysate by WB following IP.
Lane 1: ab99314 at 3µg/mg of lysate.
Lane 2: IP with an antibody which recognizes an downstream epitope of ARPC1B.
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds.
All lanes : Anti-ARPC1B antibody (ab99314) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : NIH3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 3 minutes
ab99314 被引用在 3 文献中.
- Bertier L et al. Nanobodies targeting cortactin proline rich, helical and actin binding regions downregulate invadopodium formation and matrix degradation in SCC-61 cancer cells. Biomed Pharmacother 102:230-241 (2018). PubMed: 29567535
- Bertier L et al. Inhibitory cortactin nanobodies delineate the role of NTA- and SH3-domain-specific functions during invadopodium formation and cancer cell invasion. FASEB J 31:2460-2476 (2017). PubMed: 28235780
- Chorev DS et al. Regulation of focal adhesion formation by a vinculin-Arp2/3 hybrid complex. Nat Commun 5:3758 (2014). PubMed: 24781749